Revisiting AFLP fingerprinting for an unbiased assessment of genetic structure and differentiation of taurine and zebu cattle

Autor(es)

Utsunomiya, Yuri Tani ^[1] ; Bomba, Lorenzo ^[2] ; Lucente, Giordana ^[2] ; Colli, Licia ^{[2, 3]} ; Negrini, Riccardo ^[2] ; Lenstra, Johannes Arjen ^[4] ; Erhardt, Georg ^[5] ; Garcia, Jose Fernando ^{[1, 6]} ; Ajmone-Marsan, Paolo ^{[2, 3]} ; Diversity, European Cattle Genetic

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Cattol Sacro Cuore, Inst Zootech, Piacenza - Italy

^[3] Univ Cattol Sacro Cuore, BioDNA Biodivers & Ancient DNA Res Ctr, Piacenza - Italy

^[4] Univ Utrecht, Fac Vet Med, Utrecht - Netherlands

^[5] Univ Giessen, Inst Anim Breeding & Genet, D-35390 Giessen - Germany

^[6] Univ Estadual Paulista, Fac Med Vet Aracatuba, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC GENETICS; v. 15, APR 17 2014.
• Citações Web of Science: 13

Resumo

Background: Descendants from the extinct aurochs (Bos primigenius), taurine (Bos taurus) and zebu cattle (Bos indicus) were domesticated 10,000 years ago in Southwestern and Southern Asia, respectively, and colonized the world undergoing complex events of admixture and selection. Molecular data, in particular genome-wide single nucleotide polymorphism (SNP) markers, can complement historic and archaeological records to elucidate these past events. However, SNP ascertainment in cattle has been optimized for taurine breeds, imposing limitations to the study of diversity in zebu cattle. As amplified fragment length polymorphism (AFLP) markers are discovered and genotyped as the samples are assayed, this type of marker is free of ascertainment bias. In order to obtain unbiased assessments of genetic differentiation and structure in taurine and zebu cattle, we analyzed a dataset of 135 AFLP markers in 1,593 samples from 13 zebu and 58 taurine breeds, representing nine continental areas. Results: We found a geographical pattern of expected heterozygosity in European taurine breeds decreasing with the distance from the domestication centre, arguing against a large-scale introgression from European or African aurochs. Zebu cattle were found to be at least as diverse as taurine cattle. Western African zebu cattle were found to have diverged more from Indian zebu than South American zebu. Model-based clustering and ancestry informative markers analyses suggested that this is due to taurine introgression. Although a large part of South American zebu cattle also descend from taurine cows, we did not detect significant levels of taurine ancestry in these breeds, probably because of systematic backcrossing with zebu bulls. Furthermore, limited zebu introgression was found in Podolian taurine breeds in Italy. Conclusions: The assessment of cattle diversity reported here contributes an unbiased global view to genetic differentiation and structure of taurine and zebu cattle populations, which is essential for an effective conservation of the bovine genetic resources. (AU)

• Processo FAPESP: 13/12829-0 - Comparação da diversidade molecular entre bovinos de raças europeias e brasileiras, e identificação de assinaturas de seleção no genoma da raça Nelore
• Beneficiário: José Fernando Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 11/16643-2 - Estudo de associação genômica para a detecção de regiões cromossômicas relacionadas ao perímetro escrotal de bovinos Nelore
• Beneficiário: Yuri Tani Utsunomiya
• Modalidade de apoio: Bolsas no Brasil - Mestrado