Signaling Pathways Activation by Primary Endodontic Infectious Contents and Production of Inflammatory Mediators

Autor(es)

Martinho, Frederic C. ^{[1, 2]} ; Leite, Fabio R. M. ^[3] ; Chiesa, Wanderson M. M. ^[4] ; Nascimento, Gustavo G. ^[3] ; Feres, Magda ^[5] ; Gomes, Brenda P. F. A. ^[2]

Afiliação do(s) autor(es)

^[1] UNESP UNIV Estadual Paulista, Sao Jose dos Campos Dent Sch, Endodont Div, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Campinas, Endodont Div, Dept Restorat Dent, UNICAMP, Piracicaba, SP - Brazil

^[3] Univ Fed Pelotas, Sch Dent, UFPel, Dept Semiol & Clin, Periodont Div, Pelotas, RS - Brazil

^[4] Amazonas State Univ, Endodont Div, Dept Restorat Dent, Manaus, Amazonas - Brazil

^[5] Univ Fed Pelotas, Sch Dent, Dept Restorat Dent, Postgrad Div, UFPel, Pelotas, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 40, n. 4, p. 484-489, APR 2014.
• Citações Web of Science: 10

Resumo

Introduction: This study investigated the bacterial community involved in primary endodontic diseases, evaluated its ability to activate the macrophage Toll-like receptor 4 receptor through p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-B-K) signaling pathways, and determined the levels of endotoxins and interleukins (interleukin {[}IL]-6 and -10) produced by endodontic content-stimulated macrophages. Methods: Samples were taken from 21 root canals by using sterile/apyrogenic paper points. Raw 264.7 macrophages were stimulated with root canal contents. Checkerboard DNA-DNA hybridization was used for bacterial analysis and the limulus amebocyte lysate assay for endotoxin measurement; p38 MAPK and NF-KB activation was determined by Western blot analysis. IL-6 and IL-10 were measured using the enzyme-linked immunosorbent assay. Results: Bacteria and endotoxins were detected in 100% of the samples (21/21). The most frequently observed species were Parvimonas micra (16121, 76%), Fusobacterium nudeatum ssp. nucleatum (15121, 71%), and Porphyromonas endodontalis (14121, 66%). Correlations were found between endotoxins and IL-6 and IL-10 (P <.05); p38 phosphorylation had a peak at 60 minutes, and NF-KB was quickly activated after 10 minutes of stimulation. Conclusions: It was concluded that the complex bacterial community was shown to be a potent activator of TLR-4 determined by the p38 MAPK and NF-KB signaling pathways, culminating in a high antigenicity against macrophages through the levels of IL-6 and IL-10, all significantly affected by endotoxin levels. (AU)

• Processo FAPESP: 10/19136-1 - Estudo microbiológico e de endotoxinas em canais radiculares com necrose pulpar e lesão periapical e avaliação do seu potencial antigênico contra macrófagos na produção de IL1-beta e TNF-alfa durante a terapia endodôntica
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02402-9 - Investigação do conteúdo microbiológico e de endotoxinas e sua atividade antigênica em canais radiculares de dentes com pulpite irreversível e necrose pulpar na produção de citocinas pró-inflamatórias
• Beneficiário: Ariane Cássia Salustiano Marinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/17877-4 - Análise microbiológica, de endoxinas e de citocinas pró-inflamatórias em infecções endodônticas primárias com presença de lesões periapicais
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular