| Full text | |
| Author(s): |
Martinho, Frederic C.
[1, 2]
;
Leite, Fabio R. M.
[3]
;
Chiesa, Wanderson M. M.
[4]
;
Nascimento, Gustavo G.
[3]
;
Feres, Magda
[5]
;
Gomes, Brenda P. F. A.
[2]
Total Authors: 6
|
| Affiliation: | [1] UNESP UNIV Estadual Paulista, Sao Jose dos Campos Dent Sch, Endodont Div, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil
[2] Univ Estadual Campinas, Endodont Div, Dept Restorat Dent, UNICAMP, Piracicaba, SP - Brazil
[3] Univ Fed Pelotas, Sch Dent, UFPel, Dept Semiol & Clin, Periodont Div, Pelotas, RS - Brazil
[4] Amazonas State Univ, Endodont Div, Dept Restorat Dent, Manaus, Amazonas - Brazil
[5] Univ Fed Pelotas, Sch Dent, Dept Restorat Dent, Postgrad Div, UFPel, Pelotas, RS - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF ENDODONTICS; v. 40, n. 4, p. 484-489, APR 2014. |
| Web of Science Citations: | 10 |
| Abstract | |
Introduction: This study investigated the bacterial community involved in primary endodontic diseases, evaluated its ability to activate the macrophage Toll-like receptor 4 receptor through p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-B-K) signaling pathways, and determined the levels of endotoxins and interleukins (interleukin {[}IL]-6 and -10) produced by endodontic content-stimulated macrophages. Methods: Samples were taken from 21 root canals by using sterile/apyrogenic paper points. Raw 264.7 macrophages were stimulated with root canal contents. Checkerboard DNA-DNA hybridization was used for bacterial analysis and the limulus amebocyte lysate assay for endotoxin measurement; p38 MAPK and NF-KB activation was determined by Western blot analysis. IL-6 and IL-10 were measured using the enzyme-linked immunosorbent assay. Results: Bacteria and endotoxins were detected in 100% of the samples (21/21). The most frequently observed species were Parvimonas micra (16121, 76%), Fusobacterium nudeatum ssp. nucleatum (15121, 71%), and Porphyromonas endodontalis (14121, 66%). Correlations were found between endotoxins and IL-6 and IL-10 (P <.05); p38 phosphorylation had a peak at 60 minutes, and NF-KB was quickly activated after 10 minutes of stimulation. Conclusions: It was concluded that the complex bacterial community was shown to be a potent activator of TLR-4 determined by the p38 MAPK and NF-KB signaling pathways, culminating in a high antigenicity against macrophages through the levels of IL-6 and IL-10, all significantly affected by endotoxin levels. (AU) | |
| FAPESP's process: | 10/19136-1 - Microbiological and endotoxins analysis in primarily infected root canals with apical periodontitis and their antigenic potencial against macrophages to produce IL1-beta e TNF-alfa during endodontic treatment |
| Grantee: | Brenda Paula Figueiredo de Almeida Gomes |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/02402-9 - Microbiological and LPS investigation and their antigenic activity in teeth with pulpal necrosis and irreversible pulpitis in pro-inflammatory cytokine production |
| Grantee: | Ariane Cássia Salustiano Marinho |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 10/17877-4 - Microbiological analysis and quantification of endotoxins and proinflammatory cytokines from primarily infected root canals with apical periodontitis |
| Grantee: | Brenda Paula Figueiredo de Almeida Gomes |
| Support Opportunities: | Regular Research Grants |