Recombinant Leishmania infantum Heat Shock Protein 83 for the Serodiagnosis of Cutaneous, Mucosal, and Visceral Leishmaniases

Autor(es)

Celeste, Beatriz Julieta ^[1] ; Arroyo Sanchez, Maria Carmen ^[1] ; Ramos-Sanchez, Eduardo Milton ^[1] ; Castro, Luiz Guilherme M. ^[2] ; Lima Costa, Francisco Assis ^[3] ; Goto, Hiro ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Dermatol, Fac Med, BR-05403000 Sao Paulo - Brazil

^[3] Univ Fed Piaui, Dept Clin & Cirurgia Vet, Ctr Ciencias Agr, Teresina, Piaui - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Prevent Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Tropical Medicine and Hygiene; v. 90, n. 5, p. 860-865, MAY 2014.
• Citações Web of Science: 13

Resumo

Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases. (AU)

• Processo FAPESP: 11/02235-0 - Eficácia do teste sorológico ELISA com utilização de antígeno recombinante Hsp83 de Leishmania infantum para diagnóstico de leishmaniose em pacientes infectados com HIV
• Beneficiário: Hiro Goto
• Modalidade de apoio: Auxílio à Pesquisa - Regular