| Texto completo | |
| Autor(es): |
Costa-Silva, T. A.
[1]
;
Cognette, R. C.
[1]
;
Souza, C. R. F.
[1]
;
Said, S.
[1]
;
Oliveira, W. P.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Drying Technology; v. 31, n. 15, p. 1756-1763, 2013. |
| Citações Web of Science: | 8 |
| Resumo | |
Usually immobilization is a requirement for the use of enzymes as an industrial biocatalyst. In this work, endophytic fungus Cercospora kikuchii lipase was immobilized by covalent binding on agricultural by-products and microcrystalline cellulose. The enzyme support system was submitted to spouted bed drying. Lipase immobilized on microcrystalline cellulose with 1.5% of glutaraldehyde showed the best results, presenting 179.1% of the original activity after drying, followed by rice husk (173.9%), corn stover (169.8%), sugarcane bagasse (157.3%), green coconut fiber (102.3%), and corncob (99.8%). The immobilized derivatives obtained showed a decreased enzyme activity with an average of only 17.31%, whereas the enzyme in its free form lost 85.8% of its initial activity after storage for 6 months. The operational stability showed that the biocatalysts prepared retained an average of 67.2% of the initial activity after five reuse cycles. The results showed that the use of agricultural by-products as low-cost support material associated with the spouted bed drying is promising and can contribute to industrial application of biocatalysts. (AU) | |
| Processo FAPESP: | 11/00743-8 - Produção e imobilização de lipases produzidas pelo fungo endofítico Cercospora kikuchii para aplicações biotecnológicas |
| Beneficiário: | Tales Alexandre da Costa e Silva |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |