| Full text | |
| Author(s): |
Costa-Silva, T. A.
[1]
;
Cognette, R. C.
[1]
;
Souza, C. R. F.
[1]
;
Said, S.
[1]
;
Oliveira, W. P.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Drying Technology; v. 31, n. 15, p. 1756-1763, 2013. |
| Web of Science Citations: | 8 |
| Abstract | |
Usually immobilization is a requirement for the use of enzymes as an industrial biocatalyst. In this work, endophytic fungus Cercospora kikuchii lipase was immobilized by covalent binding on agricultural by-products and microcrystalline cellulose. The enzyme support system was submitted to spouted bed drying. Lipase immobilized on microcrystalline cellulose with 1.5% of glutaraldehyde showed the best results, presenting 179.1% of the original activity after drying, followed by rice husk (173.9%), corn stover (169.8%), sugarcane bagasse (157.3%), green coconut fiber (102.3%), and corncob (99.8%). The immobilized derivatives obtained showed a decreased enzyme activity with an average of only 17.31%, whereas the enzyme in its free form lost 85.8% of its initial activity after storage for 6 months. The operational stability showed that the biocatalysts prepared retained an average of 67.2% of the initial activity after five reuse cycles. The results showed that the use of agricultural by-products as low-cost support material associated with the spouted bed drying is promising and can contribute to industrial application of biocatalysts. (AU) | |
| FAPESP's process: | 11/00743-8 - Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications |
| Grantee: | Tales Alexandre da Costa e Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |