Spouted Bed Drying as a Method for Enzyme Immobilization

Author(s)

Costa-Silva, T. A. ^[1] ; Cognette, R. C. ^[1] ; Souza, C. R. F. ^[1] ; Said, S. ^[1] ; Oliveira, W. P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Drying Technology; v. 31, n. 15, p. 1756-1763, 2013.
• Web of Science Citations: 8

Abstract

Usually immobilization is a requirement for the use of enzymes as an industrial biocatalyst. In this work, endophytic fungus Cercospora kikuchii lipase was immobilized by covalent binding on agricultural by-products and microcrystalline cellulose. The enzyme support system was submitted to spouted bed drying. Lipase immobilized on microcrystalline cellulose with 1.5% of glutaraldehyde showed the best results, presenting 179.1% of the original activity after drying, followed by rice husk (173.9%), corn stover (169.8%), sugarcane bagasse (157.3%), green coconut fiber (102.3%), and corncob (99.8%). The immobilized derivatives obtained showed a decreased enzyme activity with an average of only 17.31%, whereas the enzyme in its free form lost 85.8% of its initial activity after storage for 6 months. The operational stability showed that the biocatalysts prepared retained an average of 67.2% of the initial activity after five reuse cycles. The results showed that the use of agricultural by-products as low-cost support material associated with the spouted bed drying is promising and can contribute to industrial application of biocatalysts. (AU)

• FAPESP's process: 11/00743-8 - Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications
• Grantee: Tales Alexandre da Costa e Silva
• Support Opportunities: Scholarships in Brazil - Doctorate