| Texto completo | |
| Autor(es): |
Nogueira, Adriano Barreto
[1, 2]
;
Sogayar, Mari Cleide
[3]
;
Colquhoun, Alison
[4]
;
Siqueira, Sheila Aparecida
[5]
;
Nogueira, Ariel Barreto
[5]
;
Marchiori, Paulo Euripedes
[6]
;
Teixeira, Manoel Jacobsen
[1, 2]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med, Hosp Clin, Div Neurosurg Clin, BR-05403900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Expt Surg Res, BR-05403900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Cell & Mol Therapy Ctr, BR-05403900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Cellular & Dev Biol, BR-05403900 Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med, Hosp Clin, Dept Pathol, BR-05403900 Sao Paulo - Brazil
[6] Univ Sao Paulo, Fac Med, Dept Neurol, BR-05403900 Sao Paulo - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF TRANSLATIONAL MEDICINE; v. 12, MAR 22 2014. |
| Citações Web of Science: | 25 |
| Resumo | |
Background: Prevailingly, adult mammalian neurogenesis is thought to occur in discrete, separate locations known as neurogenic niches that are best characterized in the subgranular zone (SGZ) of the dentate gyrus and in the subventricular zone (SVZ). The existence of adult human neurogenic niches is controversial. Methods: The existence of neurogenic niches was investigated with neurogenesis marker immunostaining in histologically normal human brains obtained from autopsies. Twenty-eight adult temporal lobes, specimens from limbic structures and the hypothalamus of one newborn and one adult were examined. Results: The neural stem cell marker nestin stained circumventricular organ cells and the immature neuronal marker doublecortin (DCX) stained hypothalamic and limbic structures adjacent to circumventricular organs; both markers stained a continuous structure running from the hypothalamus to the hippocampus. The cell proliferation marker Ki-67 was detected predominately in structures that form the septo-hypothalamic continuum. Nestin-expressing cells were located in the fimbria-fornix at the insertion of the choroid plexus; ependymal cells in this structure expressed the putative neural stem cell marker CD133. From the choroidal fissure in the temporal lobe, a nestin-positive cell layer spread throughout the SVZ and subpial zone. In the subpial zone, a branch of this layer reached the hippocampal sulcus and ended in the SGZ (principally in the newborn) and in the subiculum (principally in the adults). Another branch of the nestin-positive cell layer in the subpial zone returned to the optic chiasm. DCX staining was detected in the periventricular and middle hypothalamus and more densely from the mammillary body to the subiculum through the fimbria-fornix, thus running through the principal neuronal pathway from the hippocampus to the hypothalamus. The column of the fornix forms part of this pathway and appears to coincide with the zone previously identified as the human rostral migratory stream. Partial co-labeling with DCX and the neuronal marker beta III-tubulin was also observed. Conclusions: Collectively, these findings suggest the existence of an adult human neurogenic system that rises from the circumventricular organs and follows, at minimum, the circuitry of the hypothalamus and limbic system. (AU) | |
| Processo FAPESP: | 10/51634-1 - Mapeamento de nichos neurogênicos no lobo temporal humano |
| Beneficiário: | Mari Cleide Sogayar |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/08908-2 - Investigação de aspectos estruturais e dinâmicos de proteínas na biologia e fisiopatologia de doenças vasculares, tumorais, autoimunes e metabólicas: projeto para aquisição de módulo de microscopia confocal a laser |
| Beneficiário: | Francisco Rafael Martins Laurindo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |