ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

Autor(es)

Calderano, Simone ^[1] ; Godoy, Patricia ^[1] ; Soares, Daiane ^[1] ; Sant'Anna, Osvaldo Augusto ^[2] ; Schenkman, Sergio ^[3] ; Elias, M. Carolina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil

^[2] Inst Butantan, Lab Imunoquim, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Micro Imuno & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Biochemical Parasitology; v. 193, n. 2, p. 110-113, FEB 2014.
• Citações Web of Science: 8

Resumo

Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 09/53693-8 - Controle da replicacao do dna em trypanosoma cruz e trypanosoma brucei
• Beneficiário: Maria Carolina Quartim Barbosa Elias Sabbaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular