| Full text | |
| Author(s): |
Calderano, Simone
[1]
;
Godoy, Patricia
[1]
;
Soares, Daiane
[1]
;
Sant'Anna, Osvaldo Augusto
[2]
;
Schenkman, Sergio
[3]
;
Elias, M. Carolina
[1]
Total Authors: 6
|
| Affiliation: | [1] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil
[2] Inst Butantan, Lab Imunoquim, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Micro Imuno & Parasitol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecular and Biochemical Parasitology; v. 193, n. 2, p. 110-113, FEB 2014. |
| Web of Science Citations: | 8 |
| Abstract | |
Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling |
| Grantee: | Hugo Aguirre Armelin |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |