Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Direct Visualization of the Action of Triton X-100 on Giant Vesicles of Erythrocyte Membrane Lipids

Texto completo
Autor(es):
Casadei, Bruna R. [1] ; Domingues, Cleyton C. [1] ; de Paula, Eneida [1] ; Riske, Karin A. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Bioquim, Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOPHYSICAL JOURNAL; v. 106, n. 11, p. 2417-2425, JUN 3 2014.
Citações Web of Science: 16
Resumo

The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their compositions. In fact, the impact of detergents in membrane organization is still controversial. Here, we use phase contrast and fluorescence microscopy to observe giant unilamellar vesicles (GUVs) made of erythrocyte membrane lipids (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100). We clearly show that TX-100 has a restructuring action on biomembranes. Contact with TX-100 readily induces domain formation on the previously homogeneous membrane of erythro-GUVs at physiological and room temperatures. The shape and dynamics of the formed domains point to liquid-ordered/liquid-disordered (Lo/Ld) phase separation, typically found in raft-like ternary lipid mixtures. The Ld domains are then separated from the original vesicle and completely solubilized by TX-100. The insoluble vesicle left, in the Lo phase, represents around 2/3 of the original vesicle surface at room temperature and decreases to almost 1/2 at physiological temperature. This chain of events could be entirely reproduced with biomimetic GUVs of a simple ternary lipid mixture, 2:1:2 POPC/SM/chol (phosphatidylcholine/sphyngonnyelin/cholesterol), showing that this behavior will arise because of fundamental physicochemical properties of simple lipid mixtures. This work provides direct visualization of TX-100-induced domain formation followed by selective (Ld phase) solubilization in a model system with a complex biological lipid composition. (AU)

Processo FAPESP: 10/18516-5 - Caracterização de Domínios de Membranas Eritrocitárias obtidos com ou sem uso de Detergente
Beneficiário:Cleyton Crepaldi Domingues
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/10442-8 - Perturbações em bicamadas lipídicas carregadas induzidas por pulsos elétricos, peptídeos antimicrobianos e vesículas de carga oposta
Beneficiário:Karin Do Amaral Riske
Linha de fomento: Auxílio à Pesquisa - Regular