Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults

Autor(es)

Rosa, Ana Carolina G. ^[1] ; Ferreira, Helena Lage ^[2] ; Gomes, Deriane Elias ^[1] ; Taparo, Cilene Vidovix ^[1] ; Cardoso, Tereza Cristina ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, Fac Med Vet, Virol Lab, BR-16050680 Aracatuba, SP - Brazil

^[2] FZEA USP, Dept Vet Med, BR-13635900 Pirassununga, SP - Brazil

^[3] Curso Med Vet, Dept Apoio Prod & Saude Anim, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 159, n. 6, p. 1453-1457, JUN 2014.
• Citações Web of Science: 2

Resumo

In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds. (AU)

• Processo FAPESP: 10/52465-9 - Isolamento e caracterização genética do reovirus de perus (TReoV) provenientes de quadros de enterite associados a PEMS (Poult Enteritis Mortality Syndrome) no Brasil
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50845-1 - Isolamento e caracterização genética do reovírus de Peru de amostras clínicas provenientes de quadro de entrerite associados a PEMS no Brasil
• Beneficiário: Ana Carolina Guedes Rosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado