Semen parameters and seminal plasma protein and biochemical profiles of dogs with benign prostatic hyperplasia after botulinum toxin type A intraprostatic injection

Autor(es)

Motheo, Tathiana Ferguson ^[1] ; Alves, Aracelle Elisane ^[1] ; Mostachio, Giuliano Queiroz ^[1] ; Apparicio, Maricy ^[1] ; Ribeiro, Alexandre Pinto ^[2] ; de Souza, Fabiana Ferreira ^[3] ; Lopes, Maria Denise ^[3] ; Russiano Vicente, Wilter Ricardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Dept Reprod Anim, FCAV, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Fed Mato Grosso UFMT, Dept Clin Med Vet, Fac Agron & Med Vet, Cuiaba, MT - Brazil

^[3] UNESP, Dept Reprod Anim, FMVZ, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Ciência Rural; v. 44, n. 6, p. 1113-1118, JUN 2014.
• Citações Web of Science: 3

Resumo

This study aimed to determine the effects of different concentrations of botulinum toxin type A (BT-A) on semen parameters, and seminal plasma biochemical and protein profiles of dogs with benign prostatic hyperplasia (BPH). Eighteen sexually intact male dogs with BPH were randomly divided in three groups, and received an intraprostatic injection of saline solution (control group - CG), 250UI (GI) or 500UI (GII) of BT-A under transabdominal ultrasound guidance. Semen was collected at baseline, 2, 4 and 8 weeks after treatment. Semen parameters were determined and seminal plasma pH, total protein (TP), total chlorides (TC), calcium (Ca), potassium (K), and sodium (Na) concentrations were assessed. One-dimensional sodium dodecyl sulfatepolyacrilamide gel eletrophoresis (SDS-PAGE) was performed to determine seminal plasma protein profile. Sperm parameters and seminal plasma pH, TP, TC, Ca and K mean values did not change significantly at any time point and among treated groups (P>0.05). The SDS-PAGE analysis of the pooled fractions identified 31 protein bands with molecular weights ranging from 3.9 to 106.2kDA in all treatment groups during the entire evaluation period. Regardless the used dose, intraprostatic BT-A injection do not alter semen parameters and seminal plasma biochemical and protein profiles of dogs with BPH. (AU)

• Processo FAPESP: 06/59242-0 - Avaliação dos parâmetros espermáticos e caracterização dos perfis bioquímico e proteico do plasma seminal de cães (Canis familiaris - Linnaeus, 1758) com hiperplasia prostática benigna, tratados com toxina botulínica A
• Beneficiário: Tathiana Ferguson Motheo
• Modalidade de apoio: Bolsas no Brasil - Mestrado