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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genes Related to Antiviral Activity, Cell Migration, and Lysis Are Differentially Expressed in CD4(+) T Cells in Human T Cell Leukemia Virus Type 1-Associated Myelopathy/Tropical Spastic Paraparesis Patients

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Autor(es):
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Pinto, Mariana Tomazini [1, 2, 3] ; Malta, Tathiane Maistro [1, 2, 3] ; Rodrigues, Evandra Strazza [1, 2, 3] ; Pinheiro, Daniel Guariz [1, 2, 4] ; Panepucci, Rodrigo Alexandre [1, 2, 5] ; Ribeiro Malmegrim de Farias, Kelen Cristina [1, 2, 3] ; Sousa, Alessandra De Paula [1, 2] ; Takayanagui, Osvaldo Massaiti [5] ; Tanaka, Yuetsu [6] ; Covas, Dimas Tadeu [1, 2, 5] ; Kashima, Simone [1, 2, 3]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy, Ribeirao Preto - Brazil
[2] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Reg Blood Ctr Ribeirao Preto, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci, BR-14049 Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
[6] Univ Ryukyus, Grad Sch Med, Dept Immunol, Okinawa - Japan
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: AIDS Research and Human Retroviruses; v. 30, n. 6, p. 610-622, JUN 2014.
Citações Web of Science: 3
Resumo

Human T cell leukemia virus type 1 (HTLV-1) preferentially infects CD4(+) T cells and these cells play a central role in HTLV-1 infection. In this study, we investigated the global gene expression profile of circulating CD4(+) T cells from the distinct clinical status of HTLV-1-infected individuals in regard to TAX expression levels. CD4(+) T cells were isolated from asymptomatic HTLV-1 carrier (HAC) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients in order to identify genes involved in HAM/TSP development using a microarray technique. Hierarchical clustering analysis showed that healthy control (CT) and HTLV-1-infected samples clustered separately. We also observed that the HAC and HAM/TSP groups clustered separately regardless of TAX expression. The gene expression profile of CD4(+) T cells was compared among the CT, HAC, and HAM/TSP groups. The paxillin (Pxn), chemokine (C-X-C motif) receptor 4 (Cxcr4), interleukin 27 (IL27), and granzyme A (Gzma) genes were differentially expressed between the HAC and HAM/TSP groups, regardless of TAX expression. The perforin 1 (Prf1) and forkhead box P3 (Foxp3) genes were increased in the HAM/TSP group and presented a positive correlation to the expression of TAX and the proviral load (PVL). The frequency of CD4(+)FOXP3(+) regulatory T cells (Treg) was higher in HTLV-1-infected individuals. Foxp3 gene expression was positively correlated with cell lysis-related genes (Gzma, Gzmb, and Prf1). These findings suggest that CD4(+) T cell activity is distinct between the HAC and HAM/TSP groups. (AU)

Processo FAPESP: 11/21740-7 - Avaliação dos fatores de transcrição indutores da transição epitélio-mesenquimal (EMT) na biologia das células endoteliais
Beneficiário:Mariana Tomazini Pinto
Linha de fomento: Bolsas no Brasil - Doutorado