Baseline Susceptibility and Monitoring of Brazilian Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Crambidae) to Vip3Aa20 Insecticidal Protein

Autor(es)

Bernardi, Oderlei ^[1] ; Amado, Douglas ^[1] ; Sousa, Renan S. ^[1] ; Segatti, Fabiana ^[2] ; Fatoretto, Julio ^[3] ; Burd, Anthony D. ^[4] ; Omoto, Celso ^[1]

Afiliação do(s) autor(es)

^[1] Escola Super Agr Luiz de Queiroz ESALQ USP, Dept Entomol & Acarol, BR-13418900 Sao Paulo - Brazil

^[2] Syngenta Seeds Ltda, BR-38400974 Uberlandia, MG - Brazil

^[3] Syngenta Crop Protect, BR-04795900 Sao Paulo - Brazil

^[4] Syngenta Crop Protect, Greensboro, NC 27419 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Economic Entomology; v. 107, n. 2, p. 781-790, APR 2014.
• Citações Web of Science: 14

Resumo

The genetically modified maize expressing Vip3Aa20 insecticidal protein from Bacillus thuringiensis Berliner is a biotechnological option for the control of Spodoptera frugiperda (J.E. Smith) and Diatraea saccharalis (F.) in Brazil. To support an Insect Resistance Management program, we conducted studies of baseline susceptibility and monitoring of Brazilian populations of S. frugiperda and D. saccharalis to the Vip3Aa20 insecticidal protein. Neonates were exposed to Vip3Aa20 applied on artificial diet surface. Mortality and growth inhibition were assessed after 7 d. All populations were susceptible to Vip3Aa20. The LC50 ranged from 92.38 to 611.65 ng Vip3Aa20/cm(2) for 16 populations of S. frugiperda (6.6-fold variation), and between 61.18 and 367.86 ng Vip3Aa20/cm(2) for 6 populations of D. saccharalis (sixfold variation). The EC50 ranged from 21.76 to 70.09 and 48.65 to 163.60 ng Vip3Aa20/cm(2) for S. frugiperda and D. saccharalis, respectively. There was a low interpopulation variation in susceptibility to Vip3Aa20, which represents the natural geographic variation in the response, and not the variation caused by previous exposure to selection pressure. For these two pests, the diagnostic concentrations of 2,000 and 3,600 ng of Vip3Aa20/cm(2) caused high mortality. These diagnostic concentrations will be used in resistance monitoring programs in Brazil. (AU)

• Processo FAPESP: 11/22743-0 - Avaliação do risco de evolução da resistência de Spodoptera frugiperda (J. E. Smith) e Diatraea saccharalis (Fabricius) à proteína VIP3Aa20 expressa em milho geneticamente modificado no Brasil
• Beneficiário: Oderlei Bernardi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado