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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

CHRYSANTHEMUM STUNT VIROID IN BRAZIL: SURVEY, IDENTIFICATION, BIOLOGICAL AND MOLECULAR CHARACTERIZATION AND DETECTION METHODS

Autor(es):
Gobatto, D. [1] ; Chaves, A. L. R. [1] ; Harakava, R. [1] ; Marque, J. M. [2] ; Daros, J. A. [3] ; Eiras, M. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Ctr Pesquisa & Desenvolvimento Sanidade Vegetal, Inst Biol, BR-04014002 Sao Paulo - Brazil
[2] Athena Diagnost, Atibaia, SP - Brazil
[3] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, E-46071 Valencia - Spain
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PLANT PATHOLOGY; v. 96, n. 1, p. 111-119, 2014.
Citações Web of Science: 5
Resumo

In Brazil, the ornamental flowers and plants market moves in the wholesale and retail more than two billion US dollars annually, and chrysanthemum stands out as one of the most valuable commercial species. The stunting disease induced by Chrysanthemum stunt viroid (CSVd) has become a serious problem in chrysanthemum production systems worldwide. CSVd incites also colour breaking and retards flowering, but in many situations it does not induce visible symptoms, facilitating its spread in the field and passing international borders unnoticed. In Brazil there are few studies on this pathogen, with a single report of its possible occurrence in chrysanthemum in the State of Sao Paulo. In this work we aimed at surveying, identifying and characterizyng viroids in chrysanthemum crops in the State of Sao Paulo; challenging chrysanthemum varieties with a Brazilian CSVd isolate; and establishing diagnostic strategies to strengthen quarantine and indexing programs. Our survey showed that CSVd is widely disseminated in chrysanthemum crops in the State of Sao Paulo. All evaluated chrysanthemum varieties were susceptible of infection, although infection was sometimes symptomless. The complete genome sequencing of seven CSVd isolates showed high nucleotide identity when compared with other isolates from databases. Specific oligonucleotides were designed for conventional RT-PCR and RT-qPCR CSVd analysis. Results show that CSVd can be identified with a sensitivity about 100,000 times higher than PAGE. Dot-blot proved also to be highly sensitive and, consequently, most useful for large scale diagnosis. This is the first biological and molecular identification and characterization of CSVd isolates in Brazil. (AU)

Processo FAPESP: 12/20570-3 - Clonagem de um isolado brasileiro do Chrysanthemum stunt viroid e desenvolvimento de técnica molecular de diagnóstico
Beneficiário:Danielle Gobatto
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 11/02721-1 - Viróides em crisântemo: levantamento, identificação, caracterização, avaliação de fontes de resistência e desenvolvimento de métodos de diagnóstico
Beneficiário:Danielle Gobatto
Linha de fomento: Bolsas no Brasil - Mestrado