Hypertonic saline solution reduces the inflammatory response in endotoxemic rats

Autor(es)

Mariana Cardillo Theobaldo ^[1] ; Hermes Vieira Barbeiro ^[2] ; Denise Frediani Barbeiro ^[3] ; Ricardo Petroni ^[4] ; Francisco Garcia Soriano ^[5]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil

^[5] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 67, n. 12, p. 1463-1468, 2012-12-00.

Resumo

OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. (AU)

• Processo FAPESP: 06/00443-6 - Estudo das alterações gênicas e do remodelamento cardíaco na endotoxemia induzida por lipopolissacarídeo: uso do RNA de interferência como ferrementa terapêutica para as alterações
• Beneficiário: Francisco Garcia Soriano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/03338-7 - Participação da célula B-1 na resposta inflamatória ao lipopolissacarídeo
• Beneficiário: Francisco Garcia Soriano
• Modalidade de apoio: Auxílio à Pesquisa - Regular