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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hypertonic saline solution reduces the inflammatory response in endotoxemic rats

Full text
Author(s):
Mariana Cardillo Theobaldo [1] ; Hermes Vieira Barbeiro [2] ; Denise Frediani Barbeiro [3] ; Ricardo Petroni [4] ; Francisco Garcia Soriano [5]
Total Authors: 5
Affiliation:
[1] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil
[5] Universidade de São Paulo. Faculdade de Medicina. Emergency Medicine Division - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Clinics; v. 67, n. 12, p. 1463-1468, 2012-12-00.
Abstract

OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. (AU)

FAPESP's process: 06/00443-6 - Cardiac remodeling and genic changes induced by LPS: RNAi for terapeuctic use in heart dysfunction
Grantee:Francisco Garcia Soriano
Support Opportunities: Regular Research Grants
FAPESP's process: 09/03338-7 - Role of B-1 cell in the inflammatory response deflagrated by lipopolysacharide
Grantee:Francisco Garcia Soriano
Support Opportunities: Regular Research Grants