A study on clavulanic acid production by Streptomyces clavuligerus in batch, fed-batch and continuous processes

Autor(es)

A. B. Neto ^[1] ; D. B. Hirata ^[2] ; L. C. M. Cassiano Filho ^[3] ; C. Bellão ^[4] ; A. C. Badino Júnior ^[5] ; C. O. Hokka ^[6]

Afiliação do(s) autor(es)

^[1] Federal University of São Carlos. Department of Chemical Engineering - Brasil

^[2] Federal University of São Carlos. Department of Chemical Engineering - Brasil

^[3] Federal University of São Carlos. Department of Chemical Engineering - Brasil

^[4] Federal University of São Carlos. Department of Chemical Engineering - Brasil

^[5] Federal University of São Carlos. Department of Chemical Engineering - Brasil

^[6] Federal University of São Carlos. Department of Chemical Engineering - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 22, n. 4, p. 557-563, 2005-12-00.

Resumo

Clavulanic acid (CA) is a potent inhibitor of beta-lactamases, enzymes that are responsible for the hydrolysis of beta-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasing productivity. The operating conditions: temperature, aeration and agitation, were the same in all cases, 28º C, 0.5 vvm and 800 rpm, respectively. The CA concentration obtained in the fed-batch culture, 404 mg L-1, was ca twice the value obtained in the batch culture, 194 mg L-1, while 293 mg L-1 was obtained in the continuous culture. The highest productivity was obtained in the continuous cultivation, 10.6 mg L-1 h-1, as compared with 8.8 mg L-1 h-1 in the fed-batch process and 3.5 mg L-1 h-1 in the batch process, suggesting that continuous culture of Streptomyces clavuligerus is a promising strategy for clavulanic acid production. (AU)

• Processo FAPESP: 00/10054-0 - Estudo do processo de produção de ácido clavulânico por Streptomyces clavuligerus em cultivos contínuos com alta concentração celular
• Beneficiário: Álvaro de Baptista Neto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 99/03279-7 - Estudo da purificação de cefalosporina C e ácido clavulânico a partir de caldos de fermentação
• Beneficiário: Carlos Osamu Hokka
• Modalidade de apoio: Auxílio à Pesquisa - Regular