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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A study on clavulanic acid production by Streptomyces clavuligerus in batch, fed-batch and continuous processes

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Author(s):
A. B. Neto [1] ; D. B. Hirata [2] ; L. C. M. Cassiano Filho [3] ; C. Bellão [4] ; A. C. Badino Júnior [5] ; C. O. Hokka [6]
Total Authors: 6
Affiliation:
[1] Federal University of São Carlos. Department of Chemical Engineering - Brasil
[2] Federal University of São Carlos. Department of Chemical Engineering - Brasil
[3] Federal University of São Carlos. Department of Chemical Engineering - Brasil
[4] Federal University of São Carlos. Department of Chemical Engineering - Brasil
[5] Federal University of São Carlos. Department of Chemical Engineering - Brasil
[6] Federal University of São Carlos. Department of Chemical Engineering - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Journal of Chemical Engineering; v. 22, n. 4, p. 557-563, 2005-12-00.
Abstract

Clavulanic acid (CA) is a potent inhibitor of beta-lactamases, enzymes that are responsible for the hydrolysis of beta-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasing productivity. The operating conditions: temperature, aeration and agitation, were the same in all cases, 28º C, 0.5 vvm and 800 rpm, respectively. The CA concentration obtained in the fed-batch culture, 404 mg L-1, was ca twice the value obtained in the batch culture, 194 mg L-1, while 293 mg L-1 was obtained in the continuous culture. The highest productivity was obtained in the continuous cultivation, 10.6 mg L-1 h-1, as compared with 8.8 mg L-1 h-1 in the fed-batch process and 3.5 mg L-1 h-1 in the batch process, suggesting that continuous culture of Streptomyces clavuligerus is a promising strategy for clavulanic acid production. (AU)