| Texto completo | |
| Autor(es): |
Travensolo, Regiane F.
[1, 2]
;
Carareto-Alves, Lucia M.
[2]
;
Costa, Maria V. C. G.
[2]
;
Lopes, Tiago J. S.
[2]
;
Carrilho, Emanuel
[1]
;
Lemos, Eliana G. M.
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim, Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENETICS AND MOLECULAR BIOLOGY; v. 32, n. 2, p. 340-353, 2009. |
| Citações Web of Science: | 1 |
| Resumo | |
Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants. (AU) | |
| Processo FAPESP: | 00/06289-2 - Análise da expressão de genes de patogenicidade, virulência e adaptação da bactéria Xylella fastidiosa utilizando DNA microarray |
| Beneficiário: | Regiane de Fátima Travensolo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |