| Texto completo | |
| Autor(es): Mostrar menos - |
Smith, L. C.
[1]
;
Suzuki, Jr., J.
;
Goff, A. K.
[3]
;
Filion, F.
[4]
;
Therrien, J.
[5]
;
Murphy, B. D.
[6]
;
Kohan-Ghadr, H. R.
[7]
;
Lefebvre, R.
[8]
;
Brisville, A. C.
[9]
;
Buczinski, S.
[10]
;
Fecteau, G.
[11]
;
Perecin, F.
[12]
;
Meirelles, F. V.
[13]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] Univ Montreal. Fac Med Vet
[3] Univ Montreal. Fac Med Vet
[4] Univ Montreal. Fac Med Vet
[5] Univ Montreal. Fac Med Vet
[6] Univ Montreal. Fac Med Vet
[7] Univ Montreal. Fac Med Vet
[8] Univ Montreal. Fac Med Vet
[9] Univ Montreal. Fac Med Vet
[10] Univ Montreal. Fac Med Vet
[11] Univ Montreal. Fac Med Vet
[12] Univ Sao Paulo. Dept Ciencias Basicas
[13] Univ Sao Paulo. Dept Ciencias Basicas
Número total de Afiliações: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REPRODUCTION IN DOMESTIC ANIMALS; v. 47, n. 4, SI, p. 107-114, AUG 2012. |
| Citações Web of Science: | 35 |
| Resumo | |
Many of the developmental anomalies observed in cloned animals are related to foetal and placental overgrowth, a phenomenon known as the `large offspring syndrome' (LOS) in ruminants. It has been hypothesized that the epigenetic control of imprinted genes, that is, genes that are expressed in a parental-specific manner, is at the root of LOS. Our recent research has focused on understanding epigenetic alterations to imprinted genes that are associated with assisted reproductive technologies (ART), such as early embryo in vitro culture (IVC) and somatic cell nuclear transfer (SCNT) in cattle. We have sought and identified single nucleotide polymorphisms in Bos indicus DNA useful for the analysis of parental-specific alleles and their respective transcripts in tissues from hybrid embryos derived by crossing Bos indicus and Bos taurus cattle. By analysing differentially methylated regions (DMRs) of imprinted genes SNRPN, H19 and the IGF2R in cattle, we demonstrated that there is a generalized hypomethylation of the imprinted allele and the biallelic expression of embryos produced by SCNT when compared to the methylation patterns observed in vivo (artificially inseminated). Together, these results indicate that imprinting marks are erased during the reprogramming of the somatic cell nucleus during early development, indicating that such epigenetic anomalies may play a key role in mortality and morbidity of cloned animals. (AU) | |
| Processo FAPESP: | 10/19768-8 - Controle epigenético do gene Igf2R em bovinos |
| Beneficiário: | Felipe Perecin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |