Age-related association of rDNA and telomeres with the nuclear matrix in mouse hepatocytes

Autor(es)

Moraes, Alberto S. ^{[1, 2]} ; Mondin, Mateus ^[3] ; Beletti, Marcelo E. ^[1] ; Aguiar-Perecin, Margarida L. R. ^[3] ; Guaraldo, Ana M. A. ^[4] ; Mello, Maria Luiza S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia, Inst Biomed Sci, Dept Morphol, BR-38405320 Uberlandia, MG - Brazil

^[2] State Univ Campinas UNICAMP, Inst Biol, Dept Cell Biol, BR-13083863 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Genet, Super Agr Sch Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[4] State Univ Campinas UNICAMP, Inst Biol, Dept Parasitol, BR-13083863 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 34, n. 9, p. 925-931, SEP 2010.
• Citações Web of Science: 4

Resumo

Transcribed sequences have been suggested to be associated with the nuclear matrix, differing from non-transcribing sequences, which have been reported to be contained in DNA loops. However, although a dozen of genes have their expression level affected by aging, data on chromatin-nuclear matrix interactions under this physiological condition are still scarce. In the present study, liver imprints from young, adult and old mice were subjected to FISH (fluorescence in situ hybridization) for 45S rDNA and telomeric sequences, with or without a lysis treatment to produce extended chromatin fibres. There was an increased amount of 45S rDNA sequences located in DNA loops as the animals grow older, while telomeric sequences were always observed in DNA loops irrespective of the animal age. We assume that active rRNA genes associate with the nuclear matrix, while DNA loops contain silent sequences. Transcription of each 45S rDNA repeat unit is suggested to be dependent on its interaction with the nuclear matrix. (AU)

• Processo FAPESP: 06/00066-8 - Conteúdo de DNA e organização da cromatina em diferentes modelos e condições experimentais
• Beneficiário: Maria Luiza Silveira Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular