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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Insulin temporal sensitivity and its signaling pathway in the rat pineal gland

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Autor(es):
Peliciari-Garcia, Rodrigo A. [1] ; Marcal, Anderson C. [1] ; Silva, Jessica A. [1] ; Carmo-Buonfiglio, Daniella [1] ; Amaral, Fernanda G. [1] ; Afeche, Solange Castro [2] ; Cipolla-Neto, Jose [1] ; Carvalho, Carla R. O. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci 1, Sao Paulo - Brazil
[2] Butantan Inst, Pharmacol Lab, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 87, n. 5-6, p. 169-174, JUL 31 2010.
Citações Web of Science: 14
Resumo

Aims: In our previous work, we reported that the insulin potentiating effect on melatonin synthesis is regulated by a post-transcriptional mechanism. However, the major proteins of the insulin signaling pathway (ISP) and the possible pathway component recruited on the potentiating effect of insulin had not been characterized. A second question raised was whether windows of sensitivity to insulin exist in the pineal gland due to insulin rhythmic secretion pattern. Main methods: Melatonin content from norepinephrine(NE)-synchronized pineal gland cultures was quantified by high performance liquid chromatography with electrochemical detection and arylalkylamine-N-acetyltransferase (AANAT) activity was assayed by radiometry. Immunoblotting and immunoprecipitation techniques were performed to establish the ISP proteins expression and the formation of 14-3-3: AANAT complex, respectively. Key findings: The temporal insulin susceptibility protocol revealed two periods of insulin potentiating effect, one at the beginning and another one at the end of the in vitro induced ``night{''}. In some Timed-insulin Stimulation (TSs), insulin also promoted a reduction on melatonin synthesis, showing its dual action in cultured pineal glands. The major ISP components, such as IR beta, IGF-1R, IRS-1, IRS-2 and PI3K(p85), as well tyrosine phosphorylation of pp85 were characterized within pineal glands. Insulin is not involved in the 14-3-3:AANAT complex formation. The blockage of PI3K by LY 294002 reduced melatonin synthesis and AANAT activity. Significance: The present study demonstrated windows of differential insulin sensitivity, a functional ISP and the PI3K-dependent insulin potentiating effect on NE-mediated melatonin synthesis, supporting the hypothesis of a crosstalk between noradrenergic and insulin pathways in the rat pineal gland. (C) 2010 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 07/07790-6 - Papel da insulina na regulação da síntese de melatonina pela glândula pineal: características funcionais e mecanismos de ação
Beneficiário:Rodrigo Antonio Peliciari Garcia
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 04/06767-2 - O papel da melatonina no controle do metabolismo energético: interações hormonais, ações centrais e periféricas (pineal, obesidade, diabetes e envelhecimento)
Beneficiário:José Cipolla Neto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/60000-8 - Efeito da suplementação dietética com ácidos graxos de cadeia média (AGCM) sobre a secreção de insulina modulada por carbacol em ilhotas pancreáticas isoladas de ratos jovens
Beneficiário:Anderson Carlos Marçal
Linha de fomento: Bolsas no Brasil - Doutorado