Absolute measurement of androgen receptor mRNA in peripheral blood mononuclear, preputial skin and urethral mucosa cells of control individuals with phimosis using qRT-PCR

Autor(es)

Sousa e Silva, Tatiane ^[1] ; Richetti, Flavio ^[1] ; Palmeira Santos Cunha, Daniela Patricia ^[2] ; Moreira Amarante, Antonio Carlos ^[3] ; de Souza Leao, Jovelino Quintino ^[4] ; Longui, Carlos Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Fac Ciencias Med Santa Casa Sao Paulo, Dept Physiol, Mol Med Lab, BR-01221020 Sao Paulo - Brazil

^[2] Irmandade Santa Casa Misericordia ISCSP, Pediat Surg, Sao Paulo - Brazil

^[3] Hosp Pequeno Principe, Curitiba, Parana - Brazil

^[4] Hosp Darcy Vargas, Dept Urol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Arquivos Brasileiros de Endocrinologia e Metabologia; v. 55, n. 8, p. 665-668, NOV 2011.
• Citações Web of Science: 2

Resumo

Introduction: Androgen actions are exerted upon the androgen receptor (AR), and complete genital virilization of normal 46, XY individuals depends on adequate function and expression of the AR gene in a tissue-specific manner. Objective: Standardization of normal ARmRNA in androgen-sensitive tissues. Materials and methods: In this study, we determined the quantitative amounts of ARmRNA in peripheral blood mononuclear, urethral mucosa and preputial skin cells of control subjects with phimosis by using RT-PCR. Results: The mean (SD) values of AR expression in blood, urethra and prepuce were: 0.01 (0.01); 0.43 (0.32); 0.31 (0.36), respectively. Conclusion: The AR expression is low in blood and equivalent in urethral mucosa and preputial skin, which may be useful in the diagnosis of individuals with abnormal external genitalia. Arq Bras Endocrinol Metab. 2011;55(8):665-8 (AU)

• Processo FAPESP: 05/59531-9 - Determinacao da expressao do receptor androgenico por pcr em tempo real em pacientes com anormalidades genitais.
• Beneficiário: Carlos Alberto Longui
• Modalidade de apoio: Auxílio à Pesquisa - Regular