Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Autor(es)

Moreno, Luisa Z. ^{[1, 2]} ; Paixao, Renata ^[2] ; Gobbi, Debora D. S. ^[2] ; Raimundo, Daniele C. ^[2] ; Ferreira, Thais P. ^[2] ; Moreno, Andrea M. ^[2] ; Hofer, Ernesto ^[3] ; Reis, Cristhiane M. F. ^[3] ; Matte, Glavur R. ^[1] ; Matte, Maria H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Lab Prat Saude Publ, BR-01246904 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Sanidade Suina & Virol, BR-01246904 Sao Paulo - Brazil

^[3] Fdn Inst Oswaldo Cruz, Lab Zoonoses Bacterians, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF INFECTION IN DEVELOPING COUNTRIES; v. 8, n. 4, p. 416-423, APR 2014.
• Citações Web of Science: 12

Resumo

Introduction: Listeria species are susceptible to most antibiotics. However, over the last decade, increasing reports of multidrug-resistant Listeria spp. from various sources have prompted public health concerns. The objective of this study was to characterize the antibiotic susceptibility of Listeria spp. and the genetic mechanisms that confer resistance. Methodology: Forty-six Listeria spp. isolates were studied, and their minimal inhibitory concentrations of antibiotics were determined by microdilution using Sensititre standard susceptibility MIC plates. The isolates were screened for the presence of gyrA, parC, lde, lsa(A), lnu(A), and mprF by PCR, and the amplified genes were sequenced. Results: All isolates were susceptible to penicillin, ampicillin, tetracycline, erythromycin, and carbapenems. Resistance to clindamycin, daptomycin, and oxacillin was found among L. monocytogenes and L. innocua, and all species possessed at least intermediate resistance to fluoroquinolones. GyrA, parC, and mprF were detected in all isolates; however, mutations were found only in gyrA sequences. A high daptomycin MIC, as reported previously, was observed, suggesting an intrinsic resistance of Listeria spp. to daptomycin. Conclusions: These results are consistent with reports of emerging resistance in Listeria spp. and emphasize the need for further genotypic characterization of antibiotic resistance in this genus. (AU)

• Processo FAPESP: 10/19005-4 - Identificação de genes de virulência e caracterização do perfil de resistência a antimicrobianos em cepas de Listeria spp. originárias de ambiente, alimento e infecção humana
• Beneficiário: Maria Helena Matte
• Modalidade de apoio: Auxílio à Pesquisa - Regular