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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enhanced saccharification of sugarcane bagasse using soluble cellulase supplemented with immobilized beta-glucosidase

Texto completo
Autor(es):
Borges, Diogo Gontijo [1] ; Baraldo Junior, Anderson [1] ; Farinas, Cristiane Sanchez [2, 1] ; Camargo Giordano, Raquel de Lima [1] ; Tardioli, Paulo Waldir [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] Embrapa Instrumentacao, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Bioresource Technology; v. 167, p. 206-213, SEP 2014.
Citações Web of Science: 26
Resumo

The beta-glucosidase (BG) enzyme plays a vital role in the hydrolysis of lignocellulosic biomass. Supplementation of the hydrolysis reaction medium with BG can reduce inhibitory effects, leading to greater conversion. In addition, the inclusion of immobilized BG can be a useful way of increasing enzyme stability and recyclability. BG was adsorbed on polyacrylic resin activated by carboxyl groups (BG-PC) and covalently attached to glyoxyl-agarose (BG-GA). BG-PC exhibited similar behavior to soluble BG in the hydrolysis of cellobiose, while BG-GA hydrolyzed the same substrate at a lower rate. However, the thermal stability of BG-GA was higher than that of free BG. Hydrolysis of pretreated sugarcane bagasse catalyzed by soluble cellulase supplemented with immobilized BG improved the conversion by up to 40% after 96 h of reaction. Both derivatives remained stable up to the third cycle and losses of activity were less than 50% after five cycles. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/06470-3 - Aplicação de xilanases livres e imobilizadas para produção de xilooligossacarídeos de bagaço de cana-de-açúcar
Beneficiário:Raquel de Lima Camargo Giordano
Linha de fomento: Auxílio à Pesquisa - Regular