Overexpression of EMT genes in endothelial cells during endothelial mesenchymal transition (fapesp/mit)

• Processo: 10/51962-9
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de janeiro de 2011
• Data de Término da vigência: 31 de dezembro de 2012
• Área do conhecimento: Ciências Biológicas - Genética - Genética Humana e Médica
• Acordo de Cooperação: MIT
• Pesquisador responsável: Dimas Tadeu Covas
• Beneficiário: Dimas Tadeu Covas
• Pesquisador Responsável no exterior: Robert Allan Weinberg
• Instituição Parceira no exterior: Massachusetts Institute of Technology (MIT), Estados Unidos
• Instituição Sede: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brasil
• Assunto(s): Expressão gênica Células endoteliais Transição epitelial-mesenquimal
• Palavra(s)-Chave do Pesquisador: Celula Endotelial | Celula Mesenquimal | Expressao Genica | Modificacao Genica

Resumo

Endothelial Mesenchymal Transition (EndMT) is categorized as a specialized form of Epithelial Mesenchymal transition (EMT) that occurs in embryonic development during the formation of the heart valves and is considered one of the possible causes of fibrosis. There are some evidences that activated fibroblasts of the tumor stroma may derive from endothelial cells or its precursor cells that underwent an EndMT. The principal aim of this collaboration project is to investigate if endothelial cells (ECs) could generate mesenchymal cells (MSCs) through EndMT. For this purpose, EMT-related transcription factors will be overexpressed in ECs and we will verify whether they are able to induce an EndMT and, if the EndMT occurs, what is the role of these MSCs or mesenchymal-like cells in the tumor formation. In this regard, morphological, molecular and functional analysis will be conducted in these MSCs-derived ECs population. (AU)