Single embryo and oocyte lipid fingerprinting by mass spectrometry

• Processo: 10/05854-0
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Data de Início da vigência: 01 de julho de 2010
• Data de Término da vigência: 31 de dezembro de 2010
• Área do conhecimento: Interdisciplinar
• Pesquisador responsável: Marcos Nogueira Eberlin
• Beneficiário: Marcos Nogueira Eberlin
• Instituição Sede: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brasil
• Assunto(s): Embrião Espectroscopia de massa Oócitos Fosfolipídeos Publicações de divulgação científica Artigo científico
• Palavra(s)-Chave do Pesquisador: Embryo | Lipid fingerprintint | mass spectrometry | Oocyte | Phospholipid | triacylglycerol | Espectrometria de Massas

Resumo

Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption and ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipids (represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation of the biological roles of structural and reserve lipids in embryos and oocytes by MALDI-MS offers new opportunities for comprehensive lipid research in developmental biology. (AU)