Cell lineage analysis of mirror-image morphs in Lymnaea stagnalis L.

• Processo: 07/59097-2
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Data de Início da vigência: 01 de abril de 2008
• Data de Término da vigência: 31 de maio de 2008
• Área de conhecimento: Ciências Biológicas - Morfologia - Embriologia
• Pesquisador responsável: Toshie Kawano
• Beneficiário: Toshie Kawano
• Pesquisador Anfitrião: Takahiro Asami
• Instituição Sede: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brasil
• Instituição Anfitriã: Shinshu University, Japão
• Assunto(s): Lymnaea stagnalis Morfogênese Linhagem celular
• Palavra(s)-Chave do Pesquisador: Agno3 Staining | Cell Lineage | Dextral Morph | Lymnaea Stagnalis | Morphogenesis | Sinistral Morph

Resumo

Cell lineages of two morphs of Lymnaea stagnalis will be analyzed to test whether the reversal of left-right polarity by itself alters later morphogenesis. In Lymnaea stagnalis, the dextral morph is the wild type, and the sinistral morph is a genetic mutant. It has been observed that the mortality of sinistrals is higher than that of dextrals even though they share the same genomic background from the same parents. It indicates that sinistrals suffer anomalous development because of the polarity reversal but not of genetic differences. Sinistrai malformations are evident at the trochophore and veliger stages when observed by stereomicroscopy. The method of silver-nitrate staining will be used to follow the cell lineages. Our results will determine how the process of malformation starts at the early stages of development… (AU)