Differential Effects of Fluoride During Osteoblasts Mineralization in C57BL/6J and C3H/HeJ Inbred Strains of Mice

Autor(es)

Matsuda, Sandra S. ^[1] ; Silva, Thelma L. ^[1] ; Buzalaf, Marilia A. ^[1] ; Rodrigues, Antonio C. ^[1] ; de Oliveira, Rodrigo Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Biol, Fac Odontol Bauru, BR-17012901 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 161, n. 1, p. 123-129, OCT 2014.
• Citações Web of Science: 11

Resumo

The behavior of fluoride ions in biological systems has advantages and problems. On one hand, fluoride could be a mitogenic stimulus for osteoblasts. However, high concentrations of this element can cause apoptosis in rat and mouse osteoblasts. Toward an understanding of this effect, we examined the role of sodium fluoride (NaF) in two mouse calvaria osteoblasts during the mineralization process. The animals used were C3H/HeJ (C3) and C57BL/6J (B6) mice. The calvaria cells were cultured for 28 days in the presence of several doses of NaF (0, 5, 10, 25, 50, and 75 mu M), and we performed the assays: mineralized nodule measurements, alkaline phosphatase (ALP) activity, determination of type I collagen, and matrix metalloproteinase-2 (MMP-2) activity. The results showed no effects on alkaline phosphatase activity but decreased mineralized nodule formation. In B6 cells, the NaF effect was already seen with 10 mu M of NaF and a greater increase of cellular type I collagen, and MMP-2 activity was upregulated after 7 days of NaF exposure. C3 osteoblasts showed a reduction in the mineralization pattern only after 50 mu M of NaF with a slight increase of type I collagen and downregulation of MMP-2 activity during the mineralization period. In conclusion, fluoride affects the production and degradation of the extracellular matrix during early onset and probably during the mineralization period. Additionally, the genetic factors may contribute to the variation in cell response to fluoride exposure, and the differences observed between the two strains could be explained by an alteration of the bone matrix metabolism (synthesis and degradation). (AU)

• Processo FAPESP: 06/54333-7 - Efeito diferencial do flúor durante a mineralização de osteoblastos de duas espécies de camundongos com diferentes densidades ósseas
• Beneficiário: Sandra Satiko Matsuda
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/02025-2 - Expressão de metaloproteinases de matriz de osteoblastos de duas linhagens de camundongos sob efeito do fluoreto: uma abordagem comparativa in vitro.
• Beneficiário: Rodrigo Cardoso de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Regular