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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential Effects of Fluoride During Osteoblasts Mineralization in C57BL/6J and C3H/HeJ Inbred Strains of Mice

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Matsuda, Sandra S. [1] ; Silva, Thelma L. [1] ; Buzalaf, Marilia A. [1] ; Rodrigues, Antonio C. [1] ; de Oliveira, Rodrigo Cardoso [1]
Total Authors: 5
[1] Univ Sao Paulo, Dept Ciencias Biol, Fac Odontol Bauru, BR-17012901 Bauru, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 161, n. 1, p. 123-129, OCT 2014.
Web of Science Citations: 11

The behavior of fluoride ions in biological systems has advantages and problems. On one hand, fluoride could be a mitogenic stimulus for osteoblasts. However, high concentrations of this element can cause apoptosis in rat and mouse osteoblasts. Toward an understanding of this effect, we examined the role of sodium fluoride (NaF) in two mouse calvaria osteoblasts during the mineralization process. The animals used were C3H/HeJ (C3) and C57BL/6J (B6) mice. The calvaria cells were cultured for 28 days in the presence of several doses of NaF (0, 5, 10, 25, 50, and 75 mu M), and we performed the assays: mineralized nodule measurements, alkaline phosphatase (ALP) activity, determination of type I collagen, and matrix metalloproteinase-2 (MMP-2) activity. The results showed no effects on alkaline phosphatase activity but decreased mineralized nodule formation. In B6 cells, the NaF effect was already seen with 10 mu M of NaF and a greater increase of cellular type I collagen, and MMP-2 activity was upregulated after 7 days of NaF exposure. C3 osteoblasts showed a reduction in the mineralization pattern only after 50 mu M of NaF with a slight increase of type I collagen and downregulation of MMP-2 activity during the mineralization period. In conclusion, fluoride affects the production and degradation of the extracellular matrix during early onset and probably during the mineralization period. Additionally, the genetic factors may contribute to the variation in cell response to fluoride exposure, and the differences observed between the two strains could be explained by an alteration of the bone matrix metabolism (synthesis and degradation). (AU)

FAPESP's process: 10/02025-2 - Matrix metalloproteinases expression in osteoblasts of two strains of mice under the effect of fluoride: a comparative approach in vitro
Grantee:Rodrigo Cardoso de Oliveira
Support Opportunities: Regular Research Grants