Morphological effects of bacterial compounds on the testes of Eupemphix nattereri (Anura)

Autor(es)

Franco-Belussi, Lilian ^[1] ; Leite, Gabriela Baroni ^[2] ; Freitas, Juliane Silberschmidt ^[1] ; de Oliveira, Classius ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biol, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL BIOLOGY; v. 64, n. 3, p. 261-275, 2014.
• Citações Web of Science: 2

Resumo

Amphibians are susceptible to environmental pollutants and these compounds influence the development, morphology, physiology, behavior, and reproduction of these animals. Escherichia coli is common in aquatic habitats of frogs and could also damage their reproductive activity. Thus, our objective was to evaluate the effects of E. coli lipopolysaccharide on the testes of Eupemphix nattereri after 2, 6, 12, and 24 hours. As in other anurans, the testes of E. nattereri are paired structures and spermatogenesis into the locule is cystic, with mast cells in the interstitial region as well as testicular melanocytes. The administration of lipopolysaccharide decreased the absolute locular volume after 12 h while increasing the interstitial volume. In addition, lipopolysaccharide treatment decreased the absolute volume of all cell types in animals analyzed after 12 h. The amount of mast cells in the interstitial region increased after 12 h of inoculation. Acute exposure to lipopolysaccharide clearly alters testicular morphology, decreasing the volume of both locular and germ cells. However, acute treatment did not impair spermatogenesis and after 24 h, the treatment effects were minimized. Thus, this study was the first to demonstrate that lipopolysaccharide is a potential agent for causing damage to the testes of anurans. (AU)

• Processo FAPESP: 09/13925-7 - Efeitos do hormônio sexual feminino (17-beta-estradiol) sobre a morfofisiologia testicular de anuros: Lithobates catesbeianus (Ranidae) e Rhinella schneideri (Bufonidae)
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/02067-5 - Efeitos tóxicos dos herbicidas Atrazina e Roundup em Eupemphix nattereri (Leiuperidae) e Rhinella schneideri (Bufonidae): alterações morfológicas sob diferentes concentrações e condições abióticas (pH, temperatura e fotoperíodo)
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/01840-7 - Ação antiinflamatória do hormônio alfa-MSH em anuros: estudo sistêmico e in vitro
• Beneficiário: Lilian Franco Belussi
• Modalidade de apoio: Bolsas no Brasil - Doutorado