A sensitive and specific molecular tool for detection of both typical and atypical enteroaggregative Escherichia coli

Autor(es)

Andrade, Fernanda B. ^[1] ; Gomes, Tania A. T. ^[2] ; Elias, Waldir P. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Bacteriol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microbiological Methods; v. 106, p. 16-18, NOV 2014.
• Citações Web of Science: 8

Resumo

A multiplex PCR has been standardized and evaluated for the identification of both typical and atypical enteroaggregative Escherichia coil (EAEC). The assay detecting aaiA, aaiG, aggR and aatA presented 94.8% of sensitivity, 94.3% of specificity and was able to efficiently detect both groups of EAEC among E. coli isolated from stool cultures. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/19515-2 - Padronização e avaliação de PCR multiplex para o diagnóstico de Escherichia coli enteroagregativa típica e atípica
• Beneficiário: Waldir Pereira Elias Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular