Extraction of antioxidant compounds from blackberry (Rubus sp.) bagasse using supercritical CO2 assisted by ultrasound

Autor(es)

Pasquel Reategui, Jose Luis ^[1] ; da Fonseca Machado, Ana Paula ^[1] ; Barbero, Gerardo F. ^[2] ; Rezende, Camila A. ^[3] ; Martinez, Julian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Food Engn, Coll Food Engn, BR-13083862 Campinas, SP - Brazil

^[2] Univ Cadiz, Dept Analyt Chem, Fac Sci, Agrifood Campus Int Excellence CeiA3, Cadiz 11510 - Spain

^[3] Univ Estadual Campinas, Inst Chem, UNICAMP, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 94, p. 223-233, OCT 2014.
• Citações Web of Science: 61

Resumo

Supercritical carbon dioxide extraction (SFE) was performed to recover bioactive components from blackberry (Rubus sp.) industrial residues. Ultrasound was applied during the extractions in order to enhance rate and yield. Moreover, water and ethanol at different proportions were used as cosolvents to improve the extraction of polar compounds from the residues. The extraction global yields were measured at all performed conditions (pressure, temperature, ultrasound power and cosolvent). The extracts were evaluated in terms of their antioxidant activity, measured using two methods, phenolic content, monomeric anthocyanins, and anthocyanin profile. The application of ultrasound in SFE helped increasing the extraction rate at the beginning of the process, which could be observed on the extraction curves at 15 MPa, the lowest pressure applied. Scanning electron microscopy (FESEM) was used to analyze the blackberry bagasse undergoing SFE with and without ultrasounds and showed that ultrasound disturbs the cell walls, enhancing the release of the extractable compounds. The extracts have shown high antioxidant activity and phenolic contents when obtained at higher temperatures. Regarding anthocyanins, the use of water as cosolvent resulted in a significant increase. Four major anthocyanins were identified and quantified by ultra performance liquid chromatography (UPLC). (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/02203-6 - Construção de unidades de extração, formação de partículas e reações químicas com fluidos pressurizados
• Beneficiário: Julian Martínez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/22119-7 - Estudo dos mecanismos de decomposição da parede celular vegetal
• Beneficiário: Camila Alves de Rezende
• Modalidade de apoio: Auxílio à Pesquisa - Regular