Carbon nanoparticles for gene transfection in eukaryotic cell lines

Autor(es)

Zanin, H. ^[1] ; Hollanda, L. M. ^[2] ; Ceragioli, H. J. ^[1] ; Ferreira, M. S. ^[3] ; Machado, D. ^[2] ; Lancellotti, M. ^[2] ; Catharino, R. R. ^[3] ; Baranauskas, V. ^[1] ; Lobo, A. O. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Engn Eletr & Comp, Dept Semicond Instrumentos & Foton, BR-13083852 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biochem, Biotechnol Lab, BR-13083862 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Innovare Biomarkers Lab, BR-13083877 Sao Paulo - Brazil

^[4] Univ Vale Paraiba UNIVAP, Lab Biomed Nanotechnol NANOBIO, BR-12224000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 39, p. 359-370, JUN 1 2014.
• Citações Web of Science: 13

Resumo

For the first time, oxygen terminated cellulose carbon nanoparticles (CCN) was synthesised and applied in gene transfection of pIRES plasmid. The CCN was prepared from catalytic of polyaniline by chemical vapour deposition techniques. This plasmid contains one gene that encodes the green fluorescent protein (GFP) in eukaryotic cells, making them fluorescent. This new nanomaterial and pIRES plasmid formed pi-stacking when dispersed in water by magnetic stirring. The frequencies shift in zeta potential confirmed the plasmid strongly connects to the nanomaterial. In vitro tests found that this conjugation was phagocytised by NG97, NIH-3T3 and A549 cell lines making them fluorescent, which was visualised by fluorescent microscopy. Before the transfection test, we studied CCN in cell viability. Both MTT and Neutral Red uptake tests were carried out using NG97, NIH-3T3 and A549 cell lines. Further, we use metabolomics to verify if small amounts of nanomaterial would be enough to cause some cellular damage in NG97 cells. We showed two mechanisms of action by CCN-DNA complex, producing an exogenous protein by the transfected cell and metabolomic changes that contributed by better understanding of glioblastoma, being the major finding of this work. Our results suggested that this nanomaterial has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity, good transfection efficiency, and low cell damage in small amounts of nanomaterials in metabolomic tests. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/17195-3 - Nanopartículas mesoporosas de sílica como estratégia na terapeutica antitumoral
• Beneficiário: Luciana Maria de Hollanda
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores