MicroRNAs 143 and 145 may be involved in benign prostatic hyperplasia pathogenesis through regulation of target genes and proteins

Autor(es)

Viana, Nayara I. ^[1] ; Reis, Sabrina T. ^[1] ; Dip, Nelson G. ^[1] ; Morais, Denis R. ^[1] ; Moura, Caio M. ^[1] ; Silva, Iran A. ^[1] ; Katz, Betina ^[1] ; Srougi, Miguel ^{[2, 1]} ; Leite, Katia R. M. ^[1] ; Antunes, Alberto A. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Lab Med Invest LIM55, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Div Urol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Markers; v. 29, n. 3, p. E246-E252, JUL-SEP 2014.
• Citações Web of Science: 2

Resumo

Objectives: The aim of this study was to analyze the roles of miR-143 and miR-145, as well as the gene and protein expression of their targets (KRAS, ERK5, MAP3K3, and MAP4K4) in the pathogenesis of benign prostatic hyperplasia (BPH). Methods: We analyzed the specimens of 44 patients diagnosed with BPH who underwent surgical treatment. The control group consisted of prostate samples from 2 young patients who were organ donors. miRNAs and their target genes were assessed using real-time polymerase chain reaction (qRT-PCR), and protein levels were assessed by Western blotting. Results: miR-143 and miR-145 were overexpressed in, respectively, 62.5% and 73.8% of the cases. The ERK5 and MAP4K4 genes were underexpressed respectively in 59.4% and 100% of the BPH samples, whereas KRAS and MAP3K3 were overexpressed respectively in 79.4% and 61.5% of the samples. Increased protein expression was found for both KRAS (4,312.2 luminance/area) and MAP3K3 (7,461.7 luminance/area), while the ERK5 protein was more abundant in the samples from patients with prostate larger than 60 grams (p = 0.019). Conclusions: The overexpression of miR-143 and miR-145 in BPH samples suggests an association with the pathogenesis of the disease; additionally, the latter miRNA may act through the inhibition of MAP4K4. KRAS and MAP3K3 overexpression may also be associated with BPH pathogenesis. Further analyses are necessary to confirm these results. (AU)

• Processo FAPESP: 10/50786-2 - O papel dos micro rna 143 e 145 e seus genes alvo na fisiopatologia da hiperplasia benigna da prostata.
• Beneficiário: Kátia Ramos Moreira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Regular