Systemic toxic effects during early phases of topical 4-NQO-induced oral carcinogenesis in rats

Autor(es)

Ribeiro Barcessat, Ana Rita ^[1] ; Huang, Isaac ^[1] ; Rabelo, Gustavo Davi ^[2] ; Perillo Rosin, Flavia Cristina ^[1] ; Valente Ferreira, Luciana Goncalves ^[1] ; de Cerqueira Luz, Joao Gualberto ^[3] ; Correa, Luciana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Gen Pathol Dept, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Oral Pathol Dept, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Oral Surg Dept, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 43, n. 10, p. 770-777, NOV 2014.
• Citações Web of Science: 1

Resumo

BackgroundMost studies have demonstrated 4-NQO toxicity to oral epithelium during oral carcinogenesis induction, but systemic toxicity has been poorly addressed. The aim of this study was to describe the systemic effect of 4-NQO topical application during early phases of oral cancer induction. MethodsA 4-NQO propylene glycol ointment was topically applied on the rat tongue three times a week for 16weeks. Local and systemic 4-NQO toxicity was evaluated by body weight gain, hematology, and serum chemistry analyses, histopathology, and proliferating cell nuclear antigen (PCNA) immunohistochemistry. ResultsSignificant reduction in body weight gain and in white blood cell count as well as significant increase in serum ALT and AST was observed after 16weeks of 4-NQO topical application. Focal hepatic lobular necrosis, renal tubular degeneration, and decreased cellularity in the splenic white pulp were also detected. Conclusions4-NQO topical application on the tongue of rats for 16weeks seems to have caused hepatic, renal, and splenic toxicity. Potential systemic toxicity should be considered to monitor for variables that could interfere in topical oral carcinogenesis experiments. (AU)

• Processo FAPESP: 11/07437-0 - Análise da expressão de marcadores de morte celular em lesões potencialmente malignas induzidas em língua de ratos e tratadas com terapia fotodinâmica
• Beneficiário: Luciana Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular