Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure

Autor(es)

Ribeiro, Mariana Antunes ^[1] ; dos Reis, Mariana Bisarro ^[2] ; de Moraes, Leonardo Nazario ^[1] ; Briton-Jones, Christine ^[3] ; Rainho, Claudia Aparecida ^[2] ; Scarano, Wellerson Rodrigo ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Inst Biosci, Dept Genet, BR-18618970 Botucatu, SP - Brazil

^[3] Utah Fertil Ctr, Pleasant Grove, UT 84062 - USA

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 41, n. 11, p. 7063-7066, NOV 2014.
• Citações Web of Science: 4

Resumo

Quantitative real-time RT-PCR (qPCR) has proven to be a valuable molecular technique to quantify gene expression. There are few studies in the literature that describe suitable reference genes to normalize gene expression data. Studies of transcriptionally disruptive toxins, like tetrachlorodibenzo-p-dioxin (TCDD), require careful consideration of reference genes. The present study was designed to validate potential reference genes in human Sertoli cells after exposure to TCDD. 32 candidate reference genes were analyzed to determine their applicability. geNorm and NormFinder softwares were used to obtain an estimation of the expression stability of the 32 genes and to identify the most suitable genes for qPCR data normalization. (AU)

• Processo FAPESP: 11/04202-1 - Análise do padrão de metilação das regiões controladoras de imprinting e da expressão dos genes H19 e IGF-2 após exposição à 2,3,7,8-Tetraclorodibenzo-p-dioxina (TDCC) em Células de Sertoli
• Beneficiário: Mariana Antunes Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/00253-3 - Análise do padrão de metilação das regiões controladoras de imprinting dos genes H19 e IGF2 e da resposta androgênica em células de Sertoli humanas expostas ao TCDD (2,3,7,8-tetraclorodibenzeno-p-dioxina)
• Beneficiário: Wellerson Rodrigo Scarano
• Modalidade de apoio: Auxílio à Pesquisa - Regular