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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure

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Author(s):
Ribeiro, Mariana Antunes [1] ; dos Reis, Mariana Bisarro [2] ; de Moraes, Leonardo Nazario [1] ; Briton-Jones, Christine [3] ; Rainho, Claudia Aparecida [2] ; Scarano, Wellerson Rodrigo [1]
Total Authors: 6
Affiliation:
[1] UNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil
[2] UNESP Univ Estadual Paulista, Inst Biosci, Dept Genet, BR-18618970 Botucatu, SP - Brazil
[3] Utah Fertil Ctr, Pleasant Grove, UT 84062 - USA
Total Affiliations: 3
Document type: Journal article
Source: MOLECULAR BIOLOGY REPORTS; v. 41, n. 11, p. 7063-7066, NOV 2014.
Web of Science Citations: 4
Abstract

Quantitative real-time RT-PCR (qPCR) has proven to be a valuable molecular technique to quantify gene expression. There are few studies in the literature that describe suitable reference genes to normalize gene expression data. Studies of transcriptionally disruptive toxins, like tetrachlorodibenzo-p-dioxin (TCDD), require careful consideration of reference genes. The present study was designed to validate potential reference genes in human Sertoli cells after exposure to TCDD. 32 candidate reference genes were analyzed to determine their applicability. geNorm and NormFinder softwares were used to obtain an estimation of the expression stability of the 32 genes and to identify the most suitable genes for qPCR data normalization. (AU)

FAPESP's process: 11/04202-1 - Methylation pattern of the imprinting controlling regions and expression of the H19 and IGF-2 genes after exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TDCC) in Sertoli cells.
Grantee:Mariana Antunes Ribeiro
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/00253-3 - Methylation pattern of the imprinting controlling regions and expression of the H19 and IGF-2 genes and androgenic response in human Sertoli cells after exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TDCC)
Grantee:Wellerson Rodrigo Scarano
Support Opportunities: Regular Research Grants