Evaluation of Neuronal Apoptosis Precursors in an Experimental Model of Acute Normovolemic Hemodilution

Autor(es)

Frazilio, Fabricio O. ^[1] ; Otsuki, Denise Aya ^[1] ; Noel-Morgan, Jessica ^[1] ; Maximino, Jessica Ruivo ^[2] ; Oliveira, Gabriela Pintar ^[2] ; Chadi, Gerson ^[2] ; Costa Auler, Jr., Jose Otavio ^[1] ; Fantoni, Denise Tabacchi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Anesthesia & Surg Intens Care, Lab Anesthesiol LIM08, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Neurol, Neuroregenerat Ctr LIM45, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 9 SEP 25 2014.
• Citações Web of Science: 1

Resumo

Background: The effects of acute anemia on neuronal cells and the safe limits of hematocrit are not well established. The objective of this study was to evaluate neuronal pro-and anti-apoptotic Bax and Bcl-x proteins, caspase-3 and -9 activity, and DNA fragmentation after acute normovolemic hemodilution (ANH). Methods: Twenty-four pigs were anesthetized and randomized into 4 groups: Sham, ANH to 15% hematocrit (ANH15%), ANH to 10% hematocrit (ANH10%) and hypoxia (Hx). ANH was achieved by simultaneous blood withdrawal and hydroxyethyl starch infusion. Hx consisted of ventilation with a 6% inspired oxygen fraction for 60 minutes. Bax and Bcl-x proteins as well as DNA fragmentation were evaluated in cortical nuclear and mitochondrial fractions. Caspase-3 and -9 activity was evaluated in the cortical mitochondrial and hippocampal cytosolic fractions. The data were compared using analysis of variance followed by Tukey's test (P<0.05). Results: No changes were observed in Bax protein expression after hemodilution in the ANH15% and ANH10% groups compared to the Sham group. Bax expression in the Hx group was increased in the nuclear and mitochondrial fractions compared to all other groups. No significant difference was observed in Bcl-x expression. Caspase-3 and -9 activity in the cytosolic and mitochondrial fractions was different in the Hx group compared to all other groups. No statistical significance in DNA fragmentation was found among the Sham, ANH15% or ANH10% groups. Conclusion: ANH to 10 and 15% hematocrit did not induce alterations in apoptosis precursors, suggesting that cerebral oxygenation was preserved during these anemic states. (AU)

• Processo FAPESP: 08/50060-1 - Avaliacao dos precursores da apoptose neuronal em preparados citosolicos, mitocondriais e nucleares do cortex cerebral frontal e hipocampo de porcos submetidos a hemodiluicao normovolemica aguda: e..
• Beneficiário: José Otávio Costa Auler Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/52486-9 - Avaliacao dos precursores da apoptose neuronal em preparados citosolicos, mitocondriais e nucleares do cortex cerebral frontal e hipocampo de porcos submetidos a hemodulacao normovolemica aguda...
• Beneficiário: Fabricio de Oliveira Frazilio
• Modalidade de apoio: Bolsas no Brasil - Doutorado