Histological comparison of the smooth uterine muscle of healthy golden retriever bitches, carriers of the progressive muscular dystrophy (GRMD) gene, and GRMD-affected bitches

Autor(es)

Brolio, M. P. ^[1] ; Cima, D. S. ^[2] ; Miglino, M. A. ^[1] ; Ambrosio, C. E. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FMVZ, Fac Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil

^[2] Paulista Univ UNIP, BR-02370000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, FZEA, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassunuga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 150, n. 1-2, p. 56-61, NOV 10 2014.
• Citações Web of Science: 2

Resumo

There is evidence to suggest that weakness of the pelvic and/or uterine musculature may negatively affect the obstetric performance of women who carry the gene for Duchenne muscular dystrophy (DMD). The golden retriever dog is the ideal animal model for preclinical studies of progressive muscular dystrophy, and this model is referred to as ``golden retriever muscular dystrophy (GRMD){''}. This study evaluated and compared the histopathological aspects of the uterine muscle of eleven dogs: health, n = 4; carriers of GRMD gene, n = 5; and affected females, n = 2. The obtained results showed that the uterine muscle of healthy dogs was exclusively composed of type III collagen, while a predominance of type I collagen and small amounts of type III were observed in the uterine muscle of the carriers. The myometrium of the affected bitches showed small quantities of both collagen types. The differences noted in the three evaluated groups suggest that female carrier and those individuals affected by muscular dystrophy had collagen alteration and muscle fiber commitment in the uterine muscle, a deficiency which could directly influence the composition and function of this tissue. In addition, this information is highly relevant to the reproductive management of these animals. This data open important venues for translate reproductive protocols for women, who carry the dystrophin gene. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/54517-9 - Avaliação histológica da musculatura lisa do útero de cadelas da raça Golden Retriever normais, afetadas e portadoras do gene da distrofia muscular progressiva (GRMD)
• Beneficiário: Daniela Santilli Cima
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica