Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia

Autor(es)

Korkes, Henri Augusto ^{[1, 2]} ; Sass, Nelson ^{[1, 2]} ; Moron, Antonio F. ^[1] ; Camara, Niels Olsen S. ^[3] ; Bonetti, Tatiana ^[4] ; Cerdeira, Ana Sofia ^[5] ; Cotrim Guerreiro Da Silva, Ismael Dale ^[4] ; De Oliveira, Leandro ^{[3, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Obstet, Sao Paulo - Brazil

^[2] Sch Matern Vila Nova Cachoeirinha, Lab Clin & Expt Invest, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo - Brazil

^[5] Harvard Univ, Beth Israel Deaconess Med Ctr, Dept Med, Sch Med, Boston, MA 02215 - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 10 OCT 17 2014.
• Citações Web of Science: 18

Resumo

Introduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia. Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia. Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry. Results: Approximately 200 lipid signals were quantified. The most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p, 0.0001) and PK04 (p<0.0001). Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia. (AU)

• Processo FAPESP: 12/02270-2 - Novos mecanismos celulares, moleculares e imunológicos das lesões renais agudas e crônicas: busca por novas estratégias terapêuticas
• Beneficiário: Niels Olsen Saraiva Câmara
• Modalidade de apoio: Auxílio à Pesquisa - Temático