P-31-MRS Using Visual Stimulation Protocols with Different Durations in Healthy Young Adult Subjects

Autor(es)

Barreto, Felipe R. ^{[1, 2]} ; Costa, Thiago B. S. ^{[1, 3]} ; Landim, Ricardo C. G. ^{[1, 3]} ; Castellano, Gabriela ^{[1, 3]} ; Salmon, Carlos E. G. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] CInAPCe Program Cooperacao Interinst Apoio Pesqui, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Phys, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Campinas UNICAMP, Neurophys Grp, Dept Cronol, Inst Fis Gleb Wataghin Raios Cosm Altas Energias, BR-13083859 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neurochemical Research; v. 39, n. 12, p. 2343-2350, DEC 2014.
• Citações Web of Science: 3

Resumo

Phosphorus magnetic resonance spectroscopy (P-31-MRS) combined with visual stimulation in functional experiments allows the non-invasive dynamic study of brain energy metabolism. P-31-MRS has been applied to several diseases and to healthy subjects, but works have shown variable findings and non-reproducible results, possibly caused by low numbers of subjects combined with different stimulation paradigms. In the present work, we used P-31-MRS at 3 T with two different visual stimulation protocols with different block duration ({''}short{''} and ``long{''}) to evaluate metabolic changes under different workloads in 38 healthy subjects. We found a 15 % (short protocol-blocks of 1.5 min stimulation) and 3 % (long protocol-blocks of 5 min stimulation) increase in the inorganic phosphate (Pi) to alpha-adenosine triphosphate (alpha-ATP) ratio, and a 5 % (short protocol) and 2 % (long protocol) decrease in the nicotinamide adenine nucleotide (NADH + NAD(+)) to alpha-ATP ratio. The NADH + NAD(+) results are, to the best of our knowledge, the first functional magnetic resonance spectroscopy in vivo assessment of these compounds, but their interpretation is difficult since they cannot be separately quantified at 3 T. Our results show that longer stimulations produce smaller concentration changes in Pi/alpha-ATP and (NADH + NAD(+))/alpha-ATP ratios, which suggests a possible adaptation effect during longer stimulations that leads metabolic concentrations towards the initial equilibrium. (AU)

• Processo FAPESP: 09/00270-2 - Quantificação de metabólitos cerebrais in vitro e in vivo em Espectroscopia de Ressonância Magnética
• Beneficiário: Felipe Rodrigues Barreto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/10046-2 - Espectroscopia Funcional por Ressonância Magnética (fMRS)
• Beneficiário: Gabriela Castellano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/01106-1 - Utilização da fMRS para o estudo da variação de N-acetil-aspartato e N-acetil-aspartil-glutamato durante a ativação cerebral
• Beneficiário: Ricardo Cesar Giorgetti Landim
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/56578-4 - Centro multimodal de neuroimagens para estudos em epilepsia
• Beneficiário: Fernando Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Programa CINAPCE - Temático