P-31-MRS Using Visual Stimulation Protocols with Different Durations in Healthy Young Adult Subjects

Author(s)

Barreto, Felipe R. ^{[1, 2]} ; Costa, Thiago B. S. ^{[1, 3]} ; Landim, Ricardo C. G. ^{[1, 3]} ; Castellano, Gabriela ^{[1, 3]} ; Salmon, Carlos E. G. ^{[1, 2]}

Affiliation

^[1] CInAPCe Program Cooperacao Interinst Apoio Pesqui, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Phys, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Campinas UNICAMP, Neurophys Grp, Dept Cronol, Inst Fis Gleb Wataghin Raios Cosm Altas Energias, BR-13083859 Campinas, SP - Brazil

• Document type: Journal article
• Source: Neurochemical Research; v. 39, n. 12, p. 2343-2350, DEC 2014.
• Web of Science Citations: 3

Abstract

Phosphorus magnetic resonance spectroscopy (P-31-MRS) combined with visual stimulation in functional experiments allows the non-invasive dynamic study of brain energy metabolism. P-31-MRS has been applied to several diseases and to healthy subjects, but works have shown variable findings and non-reproducible results, possibly caused by low numbers of subjects combined with different stimulation paradigms. In the present work, we used P-31-MRS at 3 T with two different visual stimulation protocols with different block duration ({''}short{''} and ``long{''}) to evaluate metabolic changes under different workloads in 38 healthy subjects. We found a 15 % (short protocol-blocks of 1.5 min stimulation) and 3 % (long protocol-blocks of 5 min stimulation) increase in the inorganic phosphate (Pi) to alpha-adenosine triphosphate (alpha-ATP) ratio, and a 5 % (short protocol) and 2 % (long protocol) decrease in the nicotinamide adenine nucleotide (NADH + NAD(+)) to alpha-ATP ratio. The NADH + NAD(+) results are, to the best of our knowledge, the first functional magnetic resonance spectroscopy in vivo assessment of these compounds, but their interpretation is difficult since they cannot be separately quantified at 3 T. Our results show that longer stimulations produce smaller concentration changes in Pi/alpha-ATP and (NADH + NAD(+))/alpha-ATP ratios, which suggests a possible adaptation effect during longer stimulations that leads metabolic concentrations towards the initial equilibrium. (AU)

• FAPESP's process: 09/00270-2 - In vitro and in vivo brain metabolites quantification using Magnetic Resonance Spectroscopy
• Grantee: Felipe Rodrigues Barreto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/10046-2 - Functional Magnetic Resonance Spectroscopy (fMRS)
• Grantee: Gabriela Castellano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/01106-1 - Use of fMRS for the study of N-acetyl-aspartate and N- acetyl-aspartyl-glutamate variation during brain activation
• Grantee: Ricardo Cesar Giorgetti Landim
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 05/56578-4 - Multimodal neuroimaging center for studies in epilepsy
• Grantee: Fernando Cendes
• Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants