Abstract
This research proposal is centered in the study of refractory mesial temporallobe epilepsy (MTLE) by high resolution structural MRI, receptor imaging and historadiological correlations. This approach will allow the development of methods for correlating in vivo and ex vivo imaging. Accordingly, we devised three projects that will investigate the basis of MRI signal alteration, serotonin transporters profile detected by molecular imaging and full analysis of brain parenchyma in patients with MTLE: A) Histological basis of MRI signal in the temporal lobe; B) Molecular imaging in mesial temporal lobe epilepsy: in vivo and ex-vivo quantification of serotonin transporters; C) Whole brain high resolution MRI analysis in patients with refractory mesial temporal lobe epilepsy. The resources (researchers, technicians, imaging equipments) involved in the accomplishment of this proposal will make available to the ClnAPCe network: 1) a multidisciplinary team able to integrate clinical and experimental epileptology, innovative imaging acquisition/processing and advanced histopathological analysis; 2) a state-of-the-art core facility for advanced imaging studies; 3) new methodologies for historadiological correlations; 4) new tools for second order imaging processing; 5) expertise on molecular imaging. These technological and scientific resources are applicable to other important areas of biomedical research (oncology, neurodegenerative and psychiatric diseases) and are designed to be integrated into a network environment sharing knowledge with other centers via e-leaming. This is an essential requirement for the developing of telemedicine, a key tool for research, education and telediagnostic activities. In spite of the importance of epilepsy to public health, the number of researchers and clinically qualified specialists in the related areas of neuroscience remains small. The ClnAPCe network initiative is expected to promote a rapid progress in this field. Consequently, we have the responsibility of disseminating knowledge generated in the network projects to the medical and scientific communities. For this reason, the main educational emphasis of our program is tumed to technology transfer in imaging processing using voxel-based morphometry (VBM), molecular imaging acquisition techniques and neuroimage databasing and data mining. Since epilepsy is a disease that causes generalized anxiety in the public at large, we intend to provide a web site devoted to increase public awareness on the scientific advances achieved by the ClnAPCe projects. (AU)
Scientific publications
(19)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIRES, FLAVIO O.;
DOS ANJOS, CARLOS A. S.;
COVOLAN, ROBERTO J. M.;
PINHEIRO, FABIANO A.;
GIBSON, ALAN ST CLAIR;
NOAKES, TIMOTHY D.;
MAGALHAESI, FERNANDO H.;
UGRINOWITSCH, CARLOS.
Cerebral Regulation in Different Maximal Aerobic Exercise Modes.
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v. 7,
JUL 5 2016.
Web of Science Citations: 5.
LANDIM, RICARDO C. G.;
EDDEN, RICHARD A. E.;
FOERSTER, BERND;
LI, LI MIN;
COVOLAN, ROBERTO J. M.;
CASTELLANO, GABRIELA.
Investigation of NAA and NAAG dynamics underlying visual stimulation using MEGA-PRESS in a functional MRS experiment.
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v. 34,
n. 3,
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APR 2016.
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COAN, ANA CAROLINA;
CAMPOS, BRUNNO M.;
BERGO, FELIPE P. G.;
KUBOTA, BRUNO Y.;
YASUDA, CLARISSA L.;
MORITA, MARCIA E.;
GUERREIRO, CARLOS A. M.;
CENDES, FERNANDO.
Patterns of seizure control in patients with mesial temporal lobe epilepsy with and without hippocampus sclerosis.
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FEB 2015.
Web of Science Citations: 7.
COAN, ANA C.;
CAMPOS, BRUNNO M.;
BELTRAMINI, GUILHERME C.;
YASUDA, CLARISSA L.;
COVOLAN, ROBERTO J. M.;
CENDES, FERNANDO.
Distinct functional and structural MRI abnormalities in mesial temporal lobe epilepsy with and without hippocampal sclerosis.
Epilepsia,
v. 55,
n. 8,
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AUG 2014.
Web of Science Citations: 16.
COAN, ANA C.;
MORITA, MARCIA E.;
CAMPOS, BRUNNO M.;
BERGO, FELIPE P. G.;
KUBOTA, BRUNO Y.;
CENDES, FERNANDO.
Amygdala enlargement occurs in patients with mesial temporal lobe epilepsy and hippocampal sclerosis with early epilepsy onset.
Epilepsy & Behavior,
v. 29,
n. 2,
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NOV 2013.
Web of Science Citations: 15.
CASSEB, R. F.;
D'ABREU, A.;
RUOCCO, H. H.;
LOPES-CENDES, I.;
CENDES, F.;
CASTELLANO, G.
Thalamic metabolic abnormalities in patients with Huntington's disease measured by magnetic resonance spectroscopy.
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Ago. 2013.
Web of Science Citations: 2.
MORITA, MARCIA E.;
YASUDA, CLARISSA LIN;
BETTING, LUIZ E.;
PACAGNELLA, DENISE;
CONZ, LIVIA;
BARBOSA, PATRICIA HORN;
MAURER-MORELLI, CLAUDIA VIANNA;
COSTA, ANDRE LUIZ F.;
KOBAYASHI, ELIANE;
LOPES-CENDES, ISCIA;
CENDES, FERNANDO.
MRI and EEG as long-term seizure outcome predictors in familial mesial temporal lobe epilepsy.
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DEC 2012.
Web of Science Citations: 15.
MAURER-MORELLI, CLAUDIA V.;
DE VASCONCELLOS, JAIRA F.;
REIS-PINTO, FERNANDA C.;
ROCHA, CRISTIANE DE S.;
DOMINGUES, ROMENIA R.;
YASUDA, CLARISSA L.;
TEDESCHI, HELDER;
DE OLIVEIRA, EVANDRO;
CENDES, FERNANDO;
LOPES-CENDES, ISCIA.
A comparison between different reference genes for expression studies in human hippocampal tissue.
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JUN 30 2012.
Web of Science Citations: 8.
PEREIRA, FABRICIO R. S.;
ALESSIO, ANDREA;
SERCHELI, MAURICIO S.;
PEDRO, TATIANE;
BILEVICIUS, ELIZABETH;
RONDINA, JANE M.;
OZELO, HELKA F. B.;
CASTELLANO, GABRIELA;
COVOLAN, ROBERTO J. M.;
DAMASCENO, BENITO P.;
CENDES, FERNANDO.
Asymmetrical hippocampal connectivity in mesial temporal lobe epilepsy: evidence from resting state fMRI.
BMC NEUROSCIENCE,
v. 11,
JUN 2 2010.
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YASUDA, CLARISSA LIN;
BETTING, LUIZ EDUARDO;
CENDES, FERNANDO.
Voxel-based morphometry and epilepsy.
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v. 10,
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JUN 2010.
Web of Science Citations: 23.