| Texto completo | |
| Autor(es): |
Cristino, Alexandre S.
[1]
;
Barchuk, Angel R.
[2]
;
Freitas, Flavia C. P.
[1, 3]
;
Narayanan, Ramesh K.
[1]
;
Biergans, Stephanie D.
[1, 4]
;
Zhao, Zhengyang
[1]
;
Simoes, Zila L. P.
[3]
;
Reinhard, Judith
[1]
;
Claudianos, Charles
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072 - Australia
[2] Univ Fed Alfenas, BR-37130000 Alfenas, MG - Brazil
[3] Univ Sao Paulo, BR-14040040 Sao Paulo - Brazil
[4] Univ Konstanz, D-78464 Constance - Germany
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NATURE COMMUNICATIONS; v. 5, NOV 2014. |
| Citações Web of Science: | 31 |
| Resumo | |
Increasing evidence suggests small non-coding RNAs (ncRNAs) such as microRNAs (miRNAs) control levels of mRNA expression during experience-related remodelling of the brain. Here we use an associative olfactory learning paradigm in the honeybee Apis mellifera to examine gene expression changes in the brain during memory formation. Brain transcriptome analysis reveals a general downregulation of protein-coding genes, including asparagine synthetase and actin, and upregulation of ncRNAs. miRNA-mRNA network predictions together with PCR validation suggest miRNAs including miR-210 and miR-932 target the downregulated protein-coding genes. Feeding cholesterol-conjugated antisense RNA to bees results in the inhibition of miR-210 and of miR-932. Loss of miR-932 impairs long-term memory formation, but not memory acquisition. Functional analyses show that miR-932 interacts with Act5C, providing evidence for direct regulation of actin expression by an miRNA. An activity-dependent increase in miR-932 expression may therefore control actin-related plasticity mechanisms and affect memory formation in the brain. (AU) | |
| Processo FAPESP: | 11/03171-5 - Análise causal do desenvolvimento de Apis mellifera: genes reguladores e redes hierárquicas de expressão gênica na especificação de tecidos e órgãos |
| Beneficiário: | Zilá Luz Paulino Simões |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 12/02061-4 - Validação das interações entre miRNAs e mRNAs-alvo envolvidas na formação do sistema nervoso em embriões fêmeos de Apis mellifera |
| Beneficiário: | Flávia Cristina de Paula Freitas |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |