Molecular basis for the improvement in muscle metaboreflex and mechanoreflex control in exercise-trained humans with chronic heart failure

Autor(es):Mostrar menos -

Antunes-Correa, Ligia M. ^[1] ; Nobre, Thais S. ^[1] ; Groehs, Raphaela V. ^[1] ; Alves, Maria Janieire N. N. ^[1] ; Fernandes, Tiago ^[2] ; Couto, Gisele K. ^[3] ; Rondon, Maria Urbana P. B. ^[2] ; Oliveira, Patricia ^[1] ; Lima, Marta ^[1] ; Mathias, Wilson ^[1] ; Brum, Patricia C. ^[2] ; Mady, Charles ^[1] ; Almeida, Dirceu R. ^[4] ; Rossoni, Luciana V. ^[3] ; Oliveira, Edilamar M. ^[2] ; Middlekauff, Holly R. ^[5] ; Negrao, Carlos E. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Heart Inst InCor, Sch Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Med, Sao Paulo - Brazil

^[5] Univ Calif Los Angeles, Geffen Sch Med, Dept Med Cardiol & Physiol, Los Angeles, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY; v. 307, n. 11, p. H1655-H1666, DEC 1 2014.
• Citações Web of Science: 27

Resumo

Previous studies have demonstrated that muscle mechanoreflex and metaboreflex controls are altered in heart failure (HF), which seems to be due to changes in cyclooxygenase (COX) pathway and changes in receptors on afferent neurons, including transient receptor potential vanilloid type-1 (TRPV1) and cannabinoid receptor type-1 (CB1). The purpose of the present study was to test the hypotheses: 1) exercise training (ET) alters the muscle metaboreflex and mechanoreflex control of muscle sympathetic nerve activity (MSNA) in HF patients. 2) The alteration in metaboreflex control is accompanied by increased expression of TRPV1 and CB1 receptors in skeletal muscle. 3) The alteration in mechanoreflex control is accompanied by COX-2 pathway in skeletal muscle. Thirty-four consecutive HF patients with ejection fractions <40% were randomized to untrained (n = 17; 54 +/- 2 yr) or exercise-trained (n = 17; 56 +/- 2 yr) groups. MSNA was recorded by microneurography. Mechanoreceptors were activated by passive exercise and metaboreceptors by postexercise circulatory arrest (PECA). COX-2 pathway, TRPV1, and CB1 receptors were measured in muscle biopsies. Following ET, resting MSNA was decreased compared with untrained group. During PECA (metaboreflex), MSNA responses were increased, which was accompanied by the expression of TRPV1 and CB1 receptors. During passive exercise (mechanoreflex), MSNA responses were decreased, which was accompanied by decreased expression of COX-2, prostaglandin-E-2 receptor-4, and thromboxane-A(2) receptor and by decreased in muscle inflammation, as indicated by increased miRNA-146 levels and the stable NF-kappa B/I kappa B-alpha ratio. In conclusion, ET alters muscle metaboreflex and mechanoreflex control of MSNA in HF patients. This alteration with ET is accompanied by alteration in TRPV1 and CB1 expression and COX-2 pathway and inflammation in skeletal muscle. (AU)

• Processo FAPESP: 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular
• Beneficiário: Carlos Eduardo Negrão
• Modalidade de apoio: Auxílio à Pesquisa - Temático