Clustering of water bodies in unpolluted and polluted environments based on Escherichia coli phylogroup abundance using a simple interaction database

Autor(es)

Stoppe, Nancy De Castro ^{[1, 2]} ; Silva, Juliana Saragiotto ^{[2, 3]} ; Torres, Tatiana Teixeira ^{[4, 2]} ; Carlos, Camila ^[1] ; Hachich, Elayse Maria ^[5] ; Zanoli Sato, Maria Ines ^[5] ; Saraiva, Antonio Mauro ^{[2, 6]} ; Mariscal Ottoboni, Laura Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Nucleo Pesquisa Biodiversidade & Computacao, Sao Paulo - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Mato Grosso, Cuiaba, MT - Brazil

^[4] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo - Brazil

^[5] Co Tecnol Saneamento Ambiental, Dept Anal Ambientais, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Escola Politecn, Dept Engn Computacao & Sistemas Digitais, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 37, n. 4, p. 694-701, 2014.
• Citações Web of Science: 3

Resumo

Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and subgroup B1 with domesticated animal sources, suggesting their use as a first screening for pollution source identification. A simple classification is also proposed based on phylogenetic subgroup distribution using the w-clique metric, enabling differentiation of polluted and unpolluted sites. (AU)

• Processo FAPESP: 07/55312-6 - Utilização de métodos moleculares no rastreamento de fontes de contaminação fecal em águas superficiais no estado de São Paulo
• Beneficiário: Laura Maria Mariscal Ottoboni
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas
• Processo FAPESP: 10/15222-0 - Prospecção de marcadores para o rastreamento de fontes de contaminação fecal em águas superficiais no Estado de São Paulo
• Beneficiário: Nancy de Castro Stoppe
• Modalidade de apoio: Bolsas no Brasil - Doutorado