Continuous Flow Synthesis of Chiral Amines in Organic Solvents: Immobilization of E. coli Cells Containing Both omega-Transaminase and PLP

Autor(es)

Andrade, Leandro H. ^[1] ; Kroutil, Wolfgang ^[2] ; Jamison, Timothy F. ^[1]

Afiliação do(s) autor(es)

^[1] MIT, Dept Chem, Cambridge, MA 02139 - USA

^[2] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz - Austria

• Tipo de documento: Artigo Científico
• Fonte: ORGANIC LETTERS; v. 16, n. 23, p. 6092-6095, DEC 5 2014.
• Citações Web of Science: 65

Resumo

E. coli cells containing overexpressed (R)-selective omega-transaminase and the cofactor PLP were immobilized on methacrylate beads suitable for continuous flow applications. The use of an organic solvent suppresses leaching of PLP from the cells; no additional cofactor was required after setting up the packed-bed reactor containing the biocatalyst (omega-TA-PLP). Non-natural ketone substrates were transformed in flow with excellent enantioselectivity (>99% ee). Features of this novel system include high-throughput (30-60 min residence time), clean production (no quench, workup, or purification required), high enzyme stability (the packed-bed reactor can be continuously operated for 1-10 days), and excellent mass recovery. (AU)

• Processo FAPESP: 13/04540-0 - Transformação de dióxido de carbono usando métodos enzimáticos sobre condições de fluxo contínuo
• Beneficiário: Leandro Helgueira de Andrade
• Modalidade de apoio: Bolsas no Exterior - Pesquisa