| Texto completo | |
| Autor(es): Mostrar menos - |
Kawahara, Rebeca
[1]
;
Granato, Daniela C.
[1]
;
Carnielli, Carolina M.
[1]
;
Cervigne, Nilva K.
[2]
;
Oliveria, Carine E.
[2]
;
Martinez, Cesar A. R.
[1]
;
Yokoo, Sami
[1]
;
Fonseca, Felipe P.
[2]
;
Lopes, Marcio
[2]
;
Santos-Silva, Alan R.
[2]
;
Graner, Edgard
[2]
;
Coletta, Ricardo D.
[2]
;
Paes Leme, Adriana Franco
[1]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] CNPEM, Lab Nacl Biociencias, Lab Espectrometria Massas, Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Fac Odontol Piracicaba, Piracicaba - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 9, n. 12 DEC 15 2014. |
| Citações Web of Science: | 22 |
| Resumo | |
Oral squamous cell carcinoma is the most common type of cancer in the oral cavity, representing more than 90% of all oral cancers. The characterization of altered molecules in oral cancer is essential to understand molecular mechanisms underlying tumor progression as well as to contribute to cancer biomarker and therapeutic target discovery. Proteoglycans are key molecular effectors of cell surface and pericellular microenvironments, performing multiple functions in cancer. Two of the major basement membrane proteoglycans, agrin and perlecan, were investigated in this study regarding their role in oral cancer. Using real time quantitative PCR (qRT-PCR), we showed that agrin and perlecan are highly expressed in oral squamous cell carcinoma. Interestingly, cell lines originated from distinct sites showed different expression of agrin and perlecan. Enzymatically targeting chondroitin sulfate modification by chondroitinase, oral squamous carcinoma cell line had a reduced ability to adhere to extracellular matrix proteins and increased sensibility to cisplatin. Additionally, knockdown of agrin and perlecan promoted a decrease on cell migration and adhesion, and on resistance of cells to cisplatin. Our study showed, for the first time, a negative regulation on oral cancer-associated events by either targeting chondroitin sulfate content or agrin and perlecan levels. (AU) | |
| Processo FAPESP: | 11/22421-2 - Determinação dos sítios de clivagens de alvos de ADAM-17 recombinante em cultura de células humanas |
| Beneficiário: | Rebeca Kawahara Sakuma |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 09/54067-3 - EMU: aquisição de um espectrômetro de massas acoplado a cromatografia líquida para permitir ampliar a capacidade de atendimento de usuários e disponibilizar novas tecnologias no Laboratório de Espectrometria de Massas do Centro de Biologia Molecular Estrutural (ABTLUS) |
| Beneficiário: | Adriana Franco Paes Leme |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 10/19278-0 - Estudo da regulação de ADAMs em câncer oral |
| Beneficiário: | Adriana Franco Paes Leme |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |